Peptide Library Construction Service

Tek Biotech is committed to providing customers with high-quality peptide library construction technology services, which will provide strong support for customers' subsequent downstream R&D work, such as peptide library screening, targeted peptide drug discovery, specific blocking peptide drug development, and specific lead sequence development. Tek Biotech has rich project experience and insights in peptide library construction. After years of development, Tek Biotech has established a perfect peptide library construction system (including but not limited to M13 phage display system relying on M13 helper phage, M13 KE phage display system, T7 phage display system, etc.), and is able to provide high-quality peptide libraries including but not limited to linear peptide libraries ( We are able to provide high-quality peptide library construction services including but not limited to linear peptide libraries (including but not limited to 6 peptide libraries, 7 peptide libraries, 12 peptide libraries, 15 peptide libraries) and cyclic peptide libraries (cyclic 6 peptide libraries, cyclic 7 peptide libraries, cyclic 10 peptide libraries, etc.). 

Peptide library construction service is based on phage display technology （Phagepro Tech^®）, which refers to the synthesis of gene libraries that meet the needs of customers by gene mutation methods such as Trimer Codon, NNK or Error-prone PCR, and then constructing them into suitable phage vectors and transforming them into the corresponding host bacteria for packaging and amplification. The phage library is then constructed into a suitable phage vector and transformed into the appropriate host bacterium for packaging and amplification. The M13 phage peptide library established by Tek Biotech has a library capacity of up to 10^8 phage peptides with a titer of up to 10^13 phage peptide particles/ml, while the T7 phage peptide library has a library capacity of up to 10^8 phage peptide particles with a titer of up to 10^11 phage peptide particles/ml, which is sufficient to support the customers' subsequent screening of peptides against a variety of targets to satisfy the needs of downstream experiments. Tek Biotech can provide one-stop technical services from peptide gene library design and synthesis, peptide library construction to peptide library screening, affinity verification, in vitro cellular validation, etc. Customers only need to provide specific project requirements, and Tek Biotech's scientists will design the optimal library construction and phage system to meet the needs of different customers' projects.

█ Peptide Library Construction Service

Tek Biotech has established two M13 phage display peptide library systems, one is the M13 phage display system that requires M13 helper phage, i.e., the synthesized peptide gene library is constructed into pEMCS vector, and the library construction is completed through transformation, amplification, and phage packaging (library rescue), etc. The other is the M13 KE phage display system that requires M13 helper phage. The second is the M13 KE phage display system, i.e., the designed and synthesized peptide gene library was constructed into M13KE phage, and the positive clones were obtained through transformation, amplification, and blue-white spot screening without the need of library rescue.The process of the M13 KE phage display peptide library construction is shown in Fig. 1:

Figure 1 Construction process of M13 KE phage display peptide library

█ Content and Periodicity of Services

█ Service Advantages

-- Multiple peptide libraries can be customized: linear peptide libraries (including, but not limited to, 6 peptides, 7 peptides, 12 peptides, 15 peptides, etc.) and cyclic peptide libraries (including, but not limited to, 6 peptides, 7 peptides, 10 peptides, etc.).

-- Various mutation systems: Trimer Codon continuous and discontinuous saturation mutation, NNK saturation mutation, Error-prone PCR mutation to construct different mutant peptide libraries to meet different customers' needs.

-- Various phage systems: M13 phage system (wild-type M13 phage system, phage system requiring assistance, etc.), T7 phage system, and so on.

-- Various vector options: pEMCS vector (phage-assisted phage system), M13 KE vector system, etc.

-- High-quality libraries: M13 phage libraries with library capacity up to 10^8, titer up to 10^13 phages/ml, library diversity >90%; T7 phage libraries with library capacity up to 10^8, titer up to 10^13 phages/ml, library diversity >90%;

-- Supporting downstream screening and validation experiments: peptide library screening (solid-phase screening, liquid-phase screening, cellular screening, in vivo animal screening, etc.), affinity validation experiments, etc.

-- One-to-one customized solutions (including peptide length and structure design, gene mutation design, library construction and screening design, as well as downstream affinity validation, blocking validation design, etc.), to meet the needs of various customers' scientific research projects.

-- Traceability of experimental records: Chinese and English experimental reports, original experimental records.