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Recombinant Antibody Expression Service

TekBiotech focuses on developing high-quality targeted drug antibodies for customers. In order to help customers conduct high-quality drugability evaluation, we have built an in vitro recombinant expression platform with mammalian cells as the core, which can provide customers with high-quality one-stop recombinant antibody preparation services including sequence analysis, linker design, fusion tag design, high expression vector design and construction, expression scheme design optimization, recombinant expression condition optimization, antibody purification, etc.

█ Antibody Recombinant Expression Service

In order to cooperate with high-quality antibody discovery services, TekBiotech has established a complete set of antibody in vitro mammalian recombinant expression and purification and strict QC control system. TekBiotech's scientists are all from biopharmaceutical companies and have many years of experience in the development of human antibody drugs. After in-depth study and understanding of GMP rules, they strictly follow the QC quality control system, ICH and GMP guidelines to develop recombinant antibody products, and provide customers with personalized and high-quality recombinant antibody customization services.

Steps

Specific Service Content

Cycle

Step 1: Gene cloning and vector construction

1) Amplify/identify gene fragments or synthesize target genes, and insert them into mammalian

cell expression plasmids

2) PCR and sequencing tests to determine subcloned genes

3) Delivery: sequencing data and experimental plan

2-3 Weeks

Step 2: Transient expression of recombinant

antibodies (small test)

1) Transfect cells (293F, CHO) with recombinant plasmids: transient expression

2) SDS-PAGE and WB detection

3) Recombinant antibody purification

4) Delivery: 1 expression vector, 1 cloning strain, 0.1-5mg recombinant antibody, purity>90%

(SDS-PAGE)

2-3 Weeks

Table 1: Recombinant antibody expression service content

█ Antibody Recombinant Expression Service Type

Using our recombinant expression technology platform, TechBio can provide customers with the following related services, as shown in Table 2.

Table 2 Types of recombinant antibody expression services

Recombinant protein mammalian expression services	Chimeric antibody expression services
Recombinant fragment antibody expression services	VHH nano antibody expression services

TekBiotech can also provide customers with one-stop technical services such as antibody humanization, CAR-T/CAR-NK lead sequence design and cell killing verification, to help customers' scientific research projects and antibody drug development.

█ Service Advantages

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Self-designed high

expression vector

One-stop technical

service

Fast cycle, high

success rate

Antibody purity

greater than 95%

Matching in vitro

verification services

Personalized

customization services

mg-g level high-throughput

expression

Recombinant Antibody Expression Service Frequently Asked Questions

What is recombinant antibody expression service?

Recombinant antibody expression service is a service based on genetic recombination technology for producing antibodies with specific antigen recognition ability. Large-scale production is carried out by inserting the gene sequence of the target antibody into a suitable expression vector and transfecting it into a suitable host cell (such as E. coli, mammalian cells, yeast, etc.). Recombinant antibodies usually have a high degree of purity and consistency and can be used for research, diagnosis or as a candidate for therapeutic drugs.
What are the criteria for selecting expression vectors for recombinant antibodies?

The selection of recombinant antibody expression vectors depends on multiple factors, including the desired antibody type (such as IgG, monoclonal antibody, single domain antibody, etc.), the compatibility of the expression system, the yield requirements, the folding and functional activity of the antibody, etc. Commonly used expression vectors include E. coli expression system, yeast expression system and mammalian cell system. Each vector has different advantages and disadvantages. Choosing the right vector can optimize the expression level and quality of the antibody.
How does the recombinant antibody expression service ensure the correct folding and activity of the antibody?

The key to ensuring the correct folding and activity of the antibody is to choose a suitable host system and optimize the expression conditions. During the expression process, the heavy and light chains of the antibody need to be correctly paired and folded to form an active dimer. In some expression systems (such as mammalian cells), the correct folding of the antibody can be ensured by providing appropriate auxiliary molecules (such as molecular chaperones). In order to further verify the functionality of the antibody, it is usually necessary to detect it through antigen binding experiments, flow cytometry and other methods to ensure that the expressed antibody has good binding affinity and biological activity.
How to evaluate the purity and quality of recombinant antibodies?

The purity and quality of recombinant antibodies are usually evaluated by a series of analytical methods. Common detection methods include: SDS-PAGE electrophoresis (analyzing the molecular weight and purity of the antibody), Western Blot (Western Blot, confirming the specificity of the antibody), affinity chromatography (used to purify the antibody and ensure its high purity), and ELISA (used to evaluate the functional activity of the antibody). In addition, mass spectrometry and amino acid sequence analysis can be used to verify whether the structure and sequence of the antibody are as expected. Each test can ensure that the recombinant antibody meets the quality standards.
What challenges may be encountered during the expression of recombinant antibodies?

Common challenges in the expression of recombinant antibodies include low expression levels, incorrect antibody folding, product aggregation or precipitation, and improper selection of expression systems. The E. coli system may cause antibodies to fail to fold correctly, resulting in insoluble inclusion bodies; while the mammalian cell system can produce correctly folded antibodies, the culture cost is high and the yield is relatively low. In addition, antibodies may be degraded or inactivated during expression due to overexpression or unstable conditions, so the expression and purification conditions need to be carefully optimized.

What is recombinant antibody expression service?

Recombinant antibody expression service is a service based on genetic recombination technology for producing antibodies with specific antigen recognition ability. Large-scale production is carried out by inserting the gene sequence of the target antibody into a suitable expression vector and transfecting it into a suitable host cell (such as E. coli, mammalian cells, yeast, etc.). Recombinant antibodies usually have a high degree of purity and consistency and can be used for research, diagnosis or as a candidate for therapeutic drugs.
What are the criteria for selecting expression vectors for recombinant antibodies?

The selection of recombinant antibody expression vectors depends on multiple factors, including the desired antibody type (such as IgG, monoclonal antibody, single domain antibody, etc.), the compatibility of the expression system, the yield requirements, the folding and functional activity of the antibody, etc. Commonly used expression vectors include E. coli expression system, yeast expression system and mammalian cell system. Each vector has different advantages and disadvantages. Choosing the right vector can optimize the expression level and quality of the antibody.
How does the recombinant antibody expression service ensure the correct folding and activity of the antibody?

The key to ensuring the correct folding and activity of the antibody is to choose a suitable host system and optimize the expression conditions. During the expression process, the heavy and light chains of the antibody need to be correctly paired and folded to form an active dimer. In some expression systems (such as mammalian cells), the correct folding of the antibody can be ensured by providing appropriate auxiliary molecules (such as molecular chaperones). In order to further verify the functionality of the antibody, it is usually necessary to detect it through antigen binding experiments, flow cytometry and other methods to ensure that the expressed antibody has good binding affinity and biological activity.
How to evaluate the purity and quality of recombinant antibodies?

The purity and quality of recombinant antibodies are usually evaluated by a series of analytical methods. Common detection methods include: SDS-PAGE electrophoresis (analyzing the molecular weight and purity of the antibody), Western Blot (Western Blot, confirming the specificity of the antibody), affinity chromatography (used to purify the antibody and ensure its high purity), and ELISA (used to evaluate the functional activity of the antibody). In addition, mass spectrometry and amino acid sequence analysis can be used to verify whether the structure and sequence of the antibody are as expected. Each test can ensure that the recombinant antibody meets the quality standards.
What challenges may be encountered during the expression of recombinant antibodies?

Common challenges in the expression of recombinant antibodies include low expression levels, incorrect antibody folding, product aggregation or precipitation, and improper selection of expression systems. The E. coli system may cause antibodies to fail to fold correctly, resulting in insoluble inclusion bodies; while the mammalian cell system can produce correctly folded antibodies, the culture cost is high and the yield is relatively low. In addition, antibodies may be degraded or inactivated during expression due to overexpression or unstable conditions, so the expression and purification conditions need to be carefully optimized.

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Recombinant Antibody Expression Service

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Recombinant Antibody Expression Service Frequently Asked Questions

What is recombinant antibody expression service?

What are the criteria for selecting expression vectors for recombinant antibodies?

How does the recombinant antibody expression service ensure the correct folding and activity of the antibody?