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Recombinant Antibody Expression Service

Tek Biotech has been focusing on recombinant antibody expression for many years, and has a professional recombinant antibody production team, which can provide customers with high-quality one-stop recombinant antibody preparation services, including sequence analysis, linker design, fusion tag design, high expression vector design and construction, expression scheme design and optimization, recombinant expression conditions optimization, antibody purification and other high-quality one-stop recombinant antibody preparation services. Antibodies are indispensable tools in immunobiology research. Recombinant antibodies have been attracting more and more attention from scientists due to their high batch-to-batch consistency, low background properties, and good animal welfare protection. Especially, in recent years, as the effectiveness of antibody drugs in the field of anti-tumor therapy has been widely recognized, recombinant antibodies are more and more valued by major pharmaceutical companies, which means that recombinant antibodies are the future of antibody research. Customers only need to provide antibody sequences and project requirements, and Tek Biotech's scientists can efficiently design recombinant antibody expression programs. Together with the perfect recombinant antibody expression system, Teckbio is able to provide a variety of high-quality recombinant antibody preparation services to meet the needs of different customers.

█ What are recombinant antibodies/proteins?

Recombinant antibodies (proteins) are antibodies (proteins) obtained by using the amino acid or base sequences of the obtained antibodies (proteins) to construct in vitro expression vectors, transfect them into suitable host cells, such as 293F, CHO cells, and express them. Antibody sequences from phage display technology screening, hybridoma sequencing and antibody full-length sequencing can be recombinantly expressed. Tek Biotech can provide customers with high quality recombinant expression services, including but not limited to VHH, scFv, Fab and antibody fusion proteins, together with Tek Biotech's self-designed high-expression vectors, multiple expression host systems (including but not limited to cell lines such as CHO-K1, CHO-S, Expi 293F, freestyle 293F, etc.), multiple specifications of the amplification expression system and complete antibody purification system. Teckbio is able to provide high-quality, university-standard recombinant antibody expression services, as well as a full traceability documentation system.

█ Recombinant Expression Technology Platform

In order to match the high quality antibody discovery service, Tek Biotech has established a complete set of in vitro lactation recombinant expression and purification of antibodies and a strict QC control system. Tek Biotech's scientists are all from biopharmaceutical companies with many years of experience in human antibody drug development. With in-depth study and understanding of GMP rules, Tek Biotech develops recombinant antibody products in strict accordance with the QC system, ICH and GMP guidelines, and provides individualized and high-quality recombinant antibody customization services for customers.

Table 1:Content of reorganization expression services

Step

Service Content

Period

Step1：Gene cloning and vector construction

* Amplification/identification of gene fragments or synthesis of target genes for insertion into mammalian cell expression plasmids

* PCR and sequencing assays to identify subcloned genes

* Delivery: sequencing data and experimental protocol

1-2 weeks

Step2: Recombinant Antibody Transient Expression

* Recombinant plasmid transfected cells (293F, CHO) : transient expression

* SDS-PAGE and WB detection

* Recombinant antibody purification

* Delivery: 1 expression vector, 1 clone strain, recombinant antibody 0.02-5mg, purity >90% (SDS-PAGE)

3-4 weeks

█ Tek Biotech Services

Utilizing our recombinant expression technology platform, Tektronix can provide the following complementary services to our customers:

Recombinant Protein 293F/CHO Cell Expression Service

Full set of Gibco serum-free mammalian cell cultures for scientists' in vitro recombinant expression needs of natural conformational antibodies. Full set of services for customers to meet the GMP-like system, including cell seed bank source documentation record system, cell seed batch verification, quality control of animal source components, standardized cell culture and transformation operation procedures, a full set of purification fillers and equipment, and other guidelines for the production of recombinant antibodies.

Chimeric Antibody Construction and Production Services

Fully customized chimeric antibody construction and lactation expression preparation services. Whether you have developed a parental antibody sequence from scratch or already have a parental antibody sequence, you can perform human-mouse chimeric antibody, human-rabbit chimeric antibody, mouse-rabbit chimeric antibody, human-alpaca chimeric antibody, etc. at Tektronix Biotechnology to meet your individual needs. For more information, please see Chimeric Antibody Construction and Production Services.

Recombinant Fragment Antibody Construction and Production Service

Fragment antibodies include single-chain antibodies scFV, VHH antibodies (camel-derived nanobodies) and Fab antibodies, etc. Customers can directly provide scFv, VHH sequences or Fab sequences, and Tektronix can design recombinant expression programs such as Fc fragment fusion antibodies of different species and different protein fusion fragment antibodies (including linker design, vector selection, label design, fusion expression program design, etc.) according to customer's needs, and then carry out recombinant expression and purification to obtain high purity products. If customers only have target antigen information, they can entrust Tektronix to conduct animal immunization and screening of scFv and VHH antibodies. Tektronix can also provide a series of complementary antibody modification (e.g., antibody humanization, antibody affinity maturation) and in vitro validation services (antibody affinity determination, antibody in vitro blocking validation, etc.).

█ Service Advantages

-- Extensive experience and well-established operational SOPs

-- Multiple expression host systems available

-- Self-designed high expression vectors

-- Stable antibody expression cell line construction service available

-- Mature recombinant antibody expression platform, strict quality control system, standardized operation procedures, to provide high-quality recombinant antibody products.

-- One-stop technical service: from sequence analysis, protocol design, gene synthesis, vector construction, recombinant expression and purification.

-- Customizable services: provide personalized services to meet the actual needs of customers.

-- Amplified expression systems of various sizes

-- Complete purification system and equipment

-- In-vitro validation of antibodies is available.

Recombinant Antibody Expression Service Frequently Asked Questions

Q: The low expression level of antibodies in the host system leads to insufficient production.

A: Optimize the construction of expression vectors, including selecting appropriate promoters, signal sequences, and host systems; Optimize cultivation conditions, such as temperature, medium composition, oxygen supply, etc., to increase expression levels; Consider using efficient transfection techniques or gene transfection methods to improve the transcription and translation efficiency of intracellular antibodies.
Q: The expressed antibodies may have issues with poor folding or incomplete assembly, affecting their structure and activity.

A: Optimize the structural design of antibodies to ensure their correct folding and assembly; Select appropriate host systems and expression conditions to promote the correct folding and assembly of antibodies; Consider using auxiliary proteins such as molecular chaperones to assist in the correct folding and assembly of antibodies.
Q: During the process of antibody expression, cells may be affected by toxicity, leading to cell death or reduction.

A: Optimize cultivation conditions to reduce the possibility of producing harmful metabolites or accumulating metabolites; Optimize the composition of cell culture medium to enhance the cell's resistance to toxicity and tolerance; Select appropriate cell lines and expression systems to reduce toxicity and mortality.
Q: The expressed antibodies may carry other proteins or impurities, resulting in low purity.

A: Optimize purification methods and steps, select appropriate purification columns, chromatography conditions, and elution buffer to improve purification efficiency; The combination of affinity chromatography, ion exchange chromatography, gel filtration and other purification methods is used to improve the purity of antibodies; Perform quality control and analysis to ensure the quality and purity of purified antibodies.
Q: The structure or properties of antigens may affect the binding efficiency and specificity of recombinant fragment antibodies.

A: Optimize the design and expression of antigens to ensure their binding efficiency and specificity with recombinant fragment antibodies; Consider using affinity modification or structural optimization methods to improve the binding efficiency and specificity between antibodies and antigens.

Q: The low expression level of antibodies in the host system leads to insufficient production.

A: Optimize the construction of expression vectors, including selecting appropriate promoters, signal sequences, and host systems; Optimize cultivation conditions, such as temperature, medium composition, oxygen supply, etc., to increase expression levels; Consider using efficient transfection techniques or gene transfection methods to improve the transcription and translation efficiency of intracellular antibodies.
Q: The expressed antibodies may have issues with poor folding or incomplete assembly, affecting their structure and activity.

A: Optimize the structural design of antibodies to ensure their correct folding and assembly; Select appropriate host systems and expression conditions to promote the correct folding and assembly of antibodies; Consider using auxiliary proteins such as molecular chaperones to assist in the correct folding and assembly of antibodies.
Q: During the process of antibody expression, cells may be affected by toxicity, leading to cell death or reduction.

A: Optimize cultivation conditions to reduce the possibility of producing harmful metabolites or accumulating metabolites; Optimize the composition of cell culture medium to enhance the cell's resistance to toxicity and tolerance; Select appropriate cell lines and expression systems to reduce toxicity and mortality.
Q: The expressed antibodies may carry other proteins or impurities, resulting in low purity.

A: Optimize purification methods and steps, select appropriate purification columns, chromatography conditions, and elution buffer to improve purification efficiency; The combination of affinity chromatography, ion exchange chromatography, gel filtration and other purification methods is used to improve the purity of antibodies; Perform quality control and analysis to ensure the quality and purity of purified antibodies.
Q: The structure or properties of antigens may affect the binding efficiency and specificity of recombinant fragment antibodies.

A: Optimize the design and expression of antigens to ensure their binding efficiency and specificity with recombinant fragment antibodies; Consider using affinity modification or structural optimization methods to improve the binding efficiency and specificity between antibodies and antigens.

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Recombinant Antibody Expression Services

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Recombinant Antibody Expression Service Frequently Asked Questions

Q: The low expression level of antibodies in the host system leads to insufficient production.

Q: The expressed antibodies may have issues with poor folding or incomplete assembly, affecting their structure and activity.

Q: During the process of antibody expression, cells may be affected by toxicity, leading to cell death or reduction.