Antibody Humanization Services

Tek Biotech has been committed to phage display technology and antibody research for many years, and has rich experience in antibody engineering and mature antibody humanization technology. Based on the phage display technology platform, Tek Biotech is able to provide chimeric antibody modification services with high affinity and low immunogenicity, as well as to humanize antibody sequences and ensure that the affinity of the modified antibody is maintained at the same order of magnitude as that of the humanized antibody, which will bring convenience to the customers for their subsequent research projects.

Figure 1 Camel-derived VHH antibody discovery service based on phage technology platforms

Antibody humanization and antibody characterization can reduce the immunogenicity of monoclonal antibodies derived from xenogeneic sources while retaining the affinity and specificity of monoclonal antibodies of parental xenogeneic sources. Replacing non-human antibody frameworks with human antibody frameworks to enhance their activation of the human immune system through DNA recombination and protein engineering technologies is a very important step in the process of therapeutic antibody discovery. To further diminish the immunogenicity of humanized antibodies, Tek Biotech conducts CDR high-throughput screening of antibody hypervariable regions for human amino acid substitutions using alanine mutation as well as combined site-directed mutagenesis. So far, Tek Biotech has successfully provided hundreds of antibody humanization services for customers.

Tek Biotech is committed to building a complete one-stop service platform for antibody humanization, providing customers with a series of services ranging from bioinformatics analysis, design of humanized antibodies, construction of high expression vectors, antibody expression and purification, affinity assay, in vitro validation of antibodies (including blocking validation), and maturation of antibody affinity in order to meet the needs of different customers.

█ Advantages of Antibody Humanization

(1) Reduced immune rejection in the body;

(2) Longer half-life in vivo than non-humanized antibodies, improving antibody pharmacokinetics;

(3) More effective recruitment of body effectors or effector cells;

(4) The affinity and specificity of the antibody are comparable to those before humanization.

█ Antibody Humanization Service Content

Tek Biotech is able to provide the following three types of customized humanized antibodies:

Hybridoma Sequencing

Customers are required to provide two tubes of hybridoma cells (5×10^6 cells/tube, frozen in cryopreservative, cell viability >90%). Tektronix will prepare mRNA by RACE and make cDNA corresponding to heavy and light chains to provide customers with sequences of variable regions, natural secretion signals and antibody isoform subclasses.

Recombinant Antibody Expression

Tek Biotech can obtain sequences of variable regions and transform them into real molecules according to the specific needs of our customers. Variable regions can be combined with original constant regions or new constant regions to produce humanized or isotype-converted antibodies. Tek Biotech can apply existing design platforms to optimize expression and balance chain ratios to maximize the yield of functional antibodies.

Tek Biotech offers a range of analytical services to analyze customers' antibodies for characterization, affinity, purity, quantity and quality. These include biostratigraphic interferometry for titer and affinity measurements using ForteBio Octets, aggregation detection by size exclusion chromatography, melting temperature and antibody stability by qPCR machines, glycan analysis by HPLC and molecular mass determination by mass spectrometry.

Antibody Humanization

Tek Biotech uses a computer modeling approach to randomize certain framework residues to CDR grafts. The grafted CDRs are cloned into a phage display library, and the phage library is screened for humanized antibodies with better affinity. To further reduce the immunogenicity of CDR-grafted humanized antibodies, Tek Biotech can reduce the murine source even more by specificity determining residue (SDR) grafting. In addition to this, Tek Biotech can also obtain humanized antibodies by direct screening from our pre-prepared humanized antibody libraries.

In addition to the above traditional antibody humanization methods, Tek Biotech also provides customers with in-depth antibody humanization services based on phage display technology platform and yeast display platform.

█ Service Content

█ Advantages of antibody humanization services

-- A scientific research team with profound expertise in antibody humanization, successfully developed many humanized antibodies.

-- Flexible antibody preparation strategy and one-to-one customization for customer needs.

-- Development of high affinity and high specificity humanized antibodies

-- High-throughput screening and flexible library screening strategy.

-- In-house development of high expression vectors

-- Bioinformatics analysis, humanized antibody design, antibody expression and purification, affinity assay, antibody affinity maturation, etc.

-- Focus on providing humanized antibodies with strong manufacturability for optimal large-scale production for clinical use