Antibody Epitope Mapping Service-Special topic on antibody modification and modification-Tekbiotech-Yeast Display Service,Phage display technology

Antibody Epitope Mapping Service

Antibodies are indispensable tools in the process of immunobiological research. Recombinant antibodies are increasingly valued by scientists due to their high batch-to-batch consistency, low background, and good protection of animal welfare characteristics. Especially, in recent years, as the effectiveness of antibody drugs in the field of anti-tumor therapy has been widely recognized, recombinant antibodies are more and more valued by major pharmaceutical companies, which means that recombinant antibodies are the future of antibody research.

Tek Biotech is a professional CRO service provider with a 5000 square meters GMP research center under construction, which can provide our clients with high quality antibody discovery and production services, such as recombinant antibody construction and expression services for scfv antibody fragments, fab antibody fragments, sdAb monodomain antibodies, rabbit monoclonal antibodies and so on.

█ Antibody Epitope Mapping Service

Epitope localization has become one of the key elements in vaccine and drug development. Epitope mapping is the process of identifying the binding site of a protein to its corresponding antigen. FDA and EMEA guidelines recommend molecular analysis of interaction sites between drugs and their regulatory filed targets. In addition, a better understanding of interaction sites can help develop more effective drugs and patent-protected products. Antibody epitope mapping is generally categorized into two types:

① Sexual epitopes: small linear peptide chain sequences of about 10-20 amino acids;

② Elephant epitopes: larger regions formed by protein folding. Since the traditional IgG antigenic determining region has a two-pocket structure, this means that an antibody usually has two types of epitopes.

Most of the therapeutic antibodies (drug antibodies) are structural antibodies, also known as spatial conformation antibodies, for which antigenic epitope mapping is commonly verified by DNA recombination techniques. The common practice is to segment the large protein into a fragment size of 50-100 amino acids, then do binding screening with the antibody, and finally obtain the antigenic sequence by LC-MS/MS.

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