Yeast Two Hybrid (Membrane System) Service

Tek Biotech is committed to providing high-quality yeast two-hybrid (membrane system) services, i.e., MbY2H services, including but not limited to the validation of interactions of known proteins, construction and screening of yeast two-hybrid (membrane system) libraries, validation of transcriptional activation of proteins based on the principles of yeast two-hybrids, and validation of the interactions of membrane proteins with membrane proteins/cytoplasmic proteins. Tek Biotech has many years of experience in the development of yeast two-hybrid projects, and is able to provide customers with a variety of customized yeast two-hybrid cDNA libraries, including, but not limited to, animal tissues, plant tissues, cells, and other samples of various species of cDNA yeast two-hybrid (membrane system) library construction services.

DUAL membrane system (DUAL membrane system) is based on split-ubiquitin-mediated membrane protein yeast two-hybrid system, which divides the ubiquitin gene into two parts to be expressed separately, i.e., the N-terminal part of the decoy protein fusion ubiquitin (NubI), and the C-terminal part of the prey protein with ubiquitin (Cub), LexA protein (which can initiate the expression of nuclear reporter genes) and VP16. If there is an interaction between the bait protein and the prey protein, the N-terminal part of ubiquitin (NubI) and the C-terminal part of ubiquitin (Cub) are close to each other, and are thus recognized by the UBPs, thus initiating the expression of the downstream reporter genes. Based on this principle, Tek Biotech has established a platform for validation of membrane protein interactions based on yeast two-hybrids (membrane system), a platform for validation of membrane protein-cytoplasmic protein interactions, and a platform for construction and screening of yeast two-hybrid cDNA libraries (which can identify many natural and potential interactions with proteins). Tek Biotech's yeast two-hybrid cDNA libraries/peptide libraries can have a library capacity of 10^6-10^7, with a library diversity and insertion rate of more than 90%, which can satisfy the quality requirements of yeast two-hybrid libraries (membrane system) of various customers. Meanwhile, Tek Biotech can also provide one-stop technical services such as downstream expression validation and affinity assay validation for yeast two-hybrid (membrane system) library construction and screening. Customers only need to provide the specific target protein sequence information, the type of library to be constructed, and sample information, and Teckbio's scientists will be able to carry out a reasonable program design and personalized customization according to the customer's needs to help the customer's research projects.

█ Yeast Two Hybrid (Membrane System) Service

Tek Biotech has established a comprehensive yeast two hybrid (membrane system) system, which can provide various high-quality customized yeast two hybrid (membrane system) services, including but not limited to cDNA library construction and screening of various species samples, known membrane protein interaction verification, known membrane protein cytoplasmic protein interaction verification, unknown interaction protein verification services, etc., to meet the scientific research needs of different customers. The service process for constructing a yeast two hybrid (membrane system) library is shown in Figure 1.

Figure 1 Yeast two-hybrid (membrane system) library construction service flow

█ Service Content and Period

█ Service Advantage

-- A wide range of samples: fresh animal tissue samples, plant tissue samples, cell samples, or total RNA from various samples (RNA must be non-degradable), etc.

-- Multiple yeast two-hybrid libraries available: cDNA yeast two-hybrid libraries of various species.

-- Proven yeast two-hybrid (membrane system) technology: yeast two-hybrid libraries up to 10^6-10^7 with >90% insertion rate and diversity.

-- High standard of delivery: yeast two-hybrid library quality assurance, providing customers with strong support for downstream experiments.

-- Traceability of experimental records: library QC standards (library capacity, diversity, insertion rate), Chinese and English lab reports, original lab records. 

-- Provide a series of downstream experimental services, such as library screening, protein expression validation and affinity validation.

-- One-to-one customized solutions, designing the best construction and screening solutions according to customer needs (e.g., selection of library construction methods (including but not limited to SMART technology, Gateway technology, etc.), vector construction design, library screening and self-activation validation solution design, etc.), to meet the needs of all kinds of customers' scientific research projects.

-- One-stop service: one-stop technical service from program design, RNA extraction, cDNA preparation, vector construction, strain transformation and screening validation.

Translated with DeepL.com (free version)