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Targeted Peptide Library Screening Service

Based on its experienced phage surface display technology, TekBiotech provides targeted peptide discovery services to global customers. Different from the recombinant M13 phage display technology we are familiar with, the M13 peptide surface display technology provided by TekBiotech is to transform the wild-type M13 phage, insert the random peptide sequence into the N-terminus of the P3 protein of the wild-type M13 phage through gene editing engineering, so that the random peptide is displayed on the surface of the wild-type M13 phage along with the expression of the coat protein, and then the targeted peptide is screened through a simple blue-white screening system.

TekBiotech is committed to providing customers with high-affinity small molecule peptide preliminary screening technology services, and providing strong support for customers' subsequent small molecule peptide cell functional verification (for example: small molecule peptide recognition and inhibition function verification in vitro, in vitro flow blocking function, etc.), in vivo functional verification (for example: small molecule peptide in vivo targeted inhibition function verification, signal pathway blocking function verification, etc.), and small molecule peptide drug development and other downstream R&D work. After 10 years of development, TekBiotech has established a complete peptide library screening system, which can provide customers with high-quality peptide library screening services (including but not limited to linear 7-peptide library, linear 12-peptide library, linear 15-peptide library, cyclic 7-peptide library, etc.) targeting targets including but not limited to proteins, peptides and various cells (including various tumor cells, primary cells, etc.).

█ Targeted Peptide Library Screening and Development Service Based On Phage Display Technology

Peptide library screening service is a screening service based on phage display technology, which refers to the use of protein, peptide, cell and other screening antigens as screening targets, and obtaining high-affinity targeted peptide sequences after multiple rounds of pressure screening. The affinity of the targeted peptides screened by TekBiotech can reach the μM-nM level, and a variety of screening methods are available for customers to choose from (including but not limited to solid phase screening, magnetic bead screening, cell screening and animal in vivo screening, etc.). The solid phase peptide library screening process is shown in Figure 1:

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Figure 1 Peptide library screening process

TekBiotech scientists will recommend the most suitable screening method based on the customer's screening targets and project requirements to obtain high-affinity peptides to meet the customer's project requirements. At the same time, TekBiotech can also provide a variety of downstream verification experiments including but not limited to peptide affinity verification (including EC50 verification, BLI affinity verification and SPR affinity verification), flow blocking verification, etc. Customers only need to provide screening targets and project requirements, and TekBiotech scientists provide one-to-one solution customization based on customer needs, saving your valuable time.

█ Targeted Peptide Phage Library Screening Service Content and Cycle

Steps

Service content

Cycle

Step1: Peptide library screening

Blue-white screening:

1)The default is 3 rounds of solid phase screening (liquid phase, solid-liquid phase, cell screening)

2)Convenient blue-white screening system, single clone sequencing, no ELISA verification required

3)Delivery: peptide sequence; screened peptide sequence information (gene information + amino acid sequence information + sequencing raw data) + project report

3-4 weeks

Step2: Peptide synthesis and affinity verification (optional)

1)Peptide synthesis

2)EC50 verification/BLI affinity verification/SPR affinity verification

3)Delivery: experimental report, raw data

3-4 weeks

█ Targeted Peptide Discovery Technology Platform Service Advantages

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Customization of various types

of peptide libraries

Mature technology platform

Various screening targets available

Personalized customization

Linear 7-peptide library, linear 12-peptide

library, linear 15-peptide library,

cyclic 7-peptide library, etc.

The affinity of the peptides obtained by

screening is generally at the μM-nM level

Proteins, peptides, cells (various tumor cells,

primary cells, etc.), etc.

Meet the scientific research project needs

of various customers

Targeted Peptide Library Screening Service Frequently Asked Questions

What is the targeted peptide library screening service? What are its main applications?

The targeted peptide library screening service is a service that uses high-throughput screening technology to select peptides with high affinity and specificity for a specific target molecule from a library containing multiple peptide sequences. Targeted peptide libraries are usually generated by chemical synthesis or biological display technology (such as phage display or yeast display), and the library contains millions to billions of different peptide sequences. The application scope of screening includes antibody drug development, targeted therapy, vaccine research, disease marker discovery and other fields. Through the screening of targeted peptide libraries, peptide candidate molecules with strong specificity and high affinity can be found for targeted drug delivery, targeted antigen recognition, vaccine design, etc.
How to perform targeted peptide library screening? What are the specific steps?

Targeted peptide library screening is usually performed through "display technology", such as phage display, yeast display, etc. The basic steps of screening are as follows: first, construct a peptide library, and each peptide in the library is bound to a display vector (such as a phage or yeast); second, incubate the library with the target (such as an antigen, receptor, etc.) to allow the peptides with specific binding ability to bind to the target; then, use elution technology to remove unbound molecules and retain only the peptides bound to the target; finally, through PCR amplification and secondary screening, further screen out high-affinity peptides, and conduct verification experiments such as affinity determination and functional testing.
What is phage display technology? How to apply it in targeted peptide library screening?

Phage display technology is a molecular screening method based on phage display. By displaying target molecules (such as peptides) on the surface of phages, a phage library is formed. Each phage carries a specific peptide sequence. By binding and screening with the target target molecule, phage display technology can help identify and screen out high-affinity and specific peptides. The specific process includes binding the phage of the peptide library to the target, washing away the unbound phage, and then screening the phage that binds to the target through PCR amplification, and further analyzing the peptide sequence displayed therein. This technology is widely used in the screening of targeted drugs, vaccine development and disease marker discovery.
How to ensure the specificity and accuracy of targeted peptide library screening?

To ensure the specificity and accuracy of targeted peptide library screening, it is necessary to first optimize the screening conditions, including selecting appropriate target concentrations, elution conditions and incubation time. In addition, the use of a suitable display system (such as phage display or yeast display) can ensure the efficient binding of peptides to targets. During the screening process, the use of highly sensitive detection technologies such as flow cytometry and surface plasmon resonance (SPR) can effectively improve the accuracy of screening. Through multiple rounds of screening and removal of non-specifically bound molecules, the background noise can be greatly reduced to ensure the specificity of the screening results. At the same time, after screening, the binding ability and biological activity of the screened peptide candidate molecules need to be further verified through affinity determination and functional experiments.
How does the high-throughput characteristics of targeted peptide library screening improve screening efficiency?

High-throughput screening is a major feature of targeted peptide library screening, which allows candidate molecules with strong binding ability to the target to be screened from a huge peptide library in a short time. By using automated equipment and microplate technology, combined with modern molecular biology techniques (such as flow cytometry, ELISA, surface plasmon resonance, etc.), thousands or even millions of samples can be quickly analyzed. High-throughput screening can significantly improve screening efficiency, reduce manual operations and sample consumption, and improve screening accuracy. During the entire screening process, by reasonably setting the screening conditions, non-specific binding can be minimized to ensure the accuracy of the screening results.

What is the targeted peptide library screening service? What are its main applications?

The targeted peptide library screening service is a service that uses high-throughput screening technology to select peptides with high affinity and specificity for a specific target molecule from a library containing multiple peptide sequences. Targeted peptide libraries are usually generated by chemical synthesis or biological display technology (such as phage display or yeast display), and the library contains millions to billions of different peptide sequences. The application scope of screening includes antibody drug development, targeted therapy, vaccine research, disease marker discovery and other fields. Through the screening of targeted peptide libraries, peptide candidate molecules with strong specificity and high affinity can be found for targeted drug delivery, targeted antigen recognition, vaccine design, etc.
How to perform targeted peptide library screening? What are the specific steps?

Targeted peptide library screening is usually performed through "display technology", such as phage display, yeast display, etc. The basic steps of screening are as follows: first, construct a peptide library, and each peptide in the library is bound to a display vector (such as a phage or yeast); second, incubate the library with the target (such as an antigen, receptor, etc.) to allow the peptides with specific binding ability to bind to the target; then, use elution technology to remove unbound molecules and retain only the peptides bound to the target; finally, through PCR amplification and secondary screening, further screen out high-affinity peptides, and conduct verification experiments such as affinity determination and functional testing.
What is phage display technology? How to apply it in targeted peptide library screening?

Phage display technology is a molecular screening method based on phage display. By displaying target molecules (such as peptides) on the surface of phages, a phage library is formed. Each phage carries a specific peptide sequence. By binding and screening with the target target molecule, phage display technology can help identify and screen out high-affinity and specific peptides. The specific process includes binding the phage of the peptide library to the target, washing away the unbound phage, and then screening the phage that binds to the target through PCR amplification, and further analyzing the peptide sequence displayed therein. This technology is widely used in the screening of targeted drugs, vaccine development and disease marker discovery.
How to ensure the specificity and accuracy of targeted peptide library screening?

To ensure the specificity and accuracy of targeted peptide library screening, it is necessary to first optimize the screening conditions, including selecting appropriate target concentrations, elution conditions and incubation time. In addition, the use of a suitable display system (such as phage display or yeast display) can ensure the efficient binding of peptides to targets. During the screening process, the use of highly sensitive detection technologies such as flow cytometry and surface plasmon resonance (SPR) can effectively improve the accuracy of screening. Through multiple rounds of screening and removal of non-specifically bound molecules, the background noise can be greatly reduced to ensure the specificity of the screening results. At the same time, after screening, the binding ability and biological activity of the screened peptide candidate molecules need to be further verified through affinity determination and functional experiments.
How does the high-throughput characteristics of targeted peptide library screening improve screening efficiency?

High-throughput screening is a major feature of targeted peptide library screening, which allows candidate molecules with strong binding ability to the target to be screened from a huge peptide library in a short time. By using automated equipment and microplate technology, combined with modern molecular biology techniques (such as flow cytometry, ELISA, surface plasmon resonance, etc.), thousands or even millions of samples can be quickly analyzed. High-throughput screening can significantly improve screening efficiency, reduce manual operations and sample consumption, and improve screening accuracy. During the entire screening process, by reasonably setting the screening conditions, non-specific binding can be minimized to ensure the accuracy of the screening results.

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Targeted Peptide Discovery Technology Service

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Targeted Peptide Library Screening Service Frequently Asked Questions

What is the targeted peptide library screening service? What are its main applications?

How to perform targeted peptide library screening? What are the specific steps?

What is phage display technology? How to apply it in targeted peptide library screening?