Yeast Two Hybrid (Nuclear System) Service

Tek Biotech is committed to providing high-quality yeast two-hybrid (nuclear system) services to our customers, providing strong support for downstream research and development, such as validation of interactions with known proteins, construction and screening of yeast two-hybrid libraries, construction and screening of peptide libraries based on the principles of yeast two-hybrids, discovery and validation of potentially interoperable proteins/small-molecule peptides, as well as the development of small peptide-targeted drugs. Tek Biotech has many years of experience in yeast two-hybrid development, and is able to provide customers with a variety of customized yeast two-hybrid services, including, but not limited to, protein interactions validation, construction of various types of yeast two-hybrid cDNA libraries (including, but not limited to, cDNA libraries of various species of samples, such as animal tissues, plant tissues, cells, etc.), construction of random peptide libraries, screening of targeting proteins/small molecules, and various other types of yeast two-hybrid services. Yeast two-hybridization (YDH) services.

Yeast two-hybrid (Y2H) is based on the principle that the DNA binding domain (BD) and active domain (AD) of the GAL4 transcription factor exert the activity of the transcription factor when they are in close spatial proximity to each other to activate the transcription and expression of the downstream genes, and the genes of the BD protein and the bait protein are constructed into the same vector. The BD protein gene and the bait protein gene were constructed into the same vector, and the AD protein gene and the prey protein gene were constructed into the same vector, and the two vectors were transferred into the same yeast strain, and if the two proteins interacted with each other so that the proximity of the BD and the AD could activate the expression of the downstream genes. Based on this principle, Tektronix has established a yeast two-hybrid-based protein interactions validation platform (since this system is an in vivo interactions validation system, the spatial conformation of the proteins is closer to the natural conformation, and the results are more authentic and reliable), and a yeast two-hybrid cDNA library construction/randomized peptide library construction and screening platform (which can discover many natural and potential interactions with proteins/peptides). Tek Biotech's yeast two-hybrid cDNA libraries/peptide libraries can have a library capacity of 10^6-10^7, with a library diversity and insertion rate of more than 90%, which can satisfy the quality requirements of yeast two-hybrid libraries of various customers. At the same time, Tek Biotech can also provide one-stop technical services such as downstream expression validation and affinity assay validation for yeast two-hybrid library construction and screening. Customers only need to provide specific target protein sequence information, library types to be constructed and sample information, and Tek Biotech's scientists will be able to carry out a reasonable program design and personalized customization according to the customer's needs for the customer's scientific research projects and targeting. Interacting protein/small molecule peptide discovery.

█ Yeast Two Hybrid (Nuclear System) Service

Tek Biotech has established a comprehensive yeast two hybrid system, which can provide various high-quality customized yeast two hybrid services, including but not limited to the construction and screening of cDNA libraries for various species samples, the construction and screening of genomic DNA random peptide libraries, the construction and screening of prefabricated random peptide libraries, the validation of known protein interactions, the validation and discovery of unknown interacting proteins, etc., to meet the scientific research needs of different customers. The service process for constructing a yeast two hybrid library is shown in Figure 1.

Figure 1 Service process for constructing yeast two hybrid library

█ Service Content and Period

█ Service Advantages

-- Multiple species samples to choose from: fresh animal tissue samples, plant tissue samples, cell samples, or total RNA of various types of samples (ensuring that RNA is not degraded), etc

-- Multiple yeast two hybrid libraries are available: construction of cDNA yeast two hybrid libraries for various species samples, construction of genomic random peptide yeast two hybrid libraries, and construction of prefabricated random peptide yeast two hybrid libraries

-- Mature yeast two hybrid technology: The yeast two hybrid library can reach a storage capacity of 10 ^ 6-10 ^ 7, with insertion rate and diversity both exceeding 90%

-- High standard delivery: yeast two hybrid library quality assurance, providing strong support for customers' downstream experiments

-- Traceability of experimental records: Library QC quality control standards (storage capacity, diversity, insertion rate), Chinese and English experimental reports, original experimental records

-- We can provide a series of downstream experimental services, including library screening, protein/peptide expression validation, and affinity validation

-- One on one personalized solution customization, designing the best construction and screening plan based on customer needs (such as the selection of library construction methods (including but not limited to SMART technology, Gateway technology, etc.), carrier construction design, library screening and self activation verification plan design, etc.), to meet the research project needs of various customers

-- One stop service: one-stop technical services including scheme design, RNA extraction, cDNA preparation, vector construction, strain transformation, screening and validation