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Recombinant IgG Customization Services

TekBiotech has a complete recombinant antibody expression production platform and rich experience in recombinant protein and recombinant antibody expression. It can provide customers with recombinant IgG antibody expression services of various species including human, mouse, rabbit, sheep, dog, camel, cattle, etc. Customers only need to provide antibody sequence information, hybridoma cells or cDNA. The company's technical support can design solutions and optimize expression systems according to customer needs. Combined with the company's independently designed high-expression vectors for the lactation system and large-scale recombinant antibody fermentation platform, it provides customers with high-quality recombinant antibody preparation services.

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Figure1 IgG antibody recombinant expression process

█ Recombinant IgG Antibody Expression Service Type

Using our recombinant expression technology platform, TekBiotech can provide customers with the following related IgG antibody recombinant expression services, as shown in Table 1.

Table 1 Recombinant antibody expression service type

Species	IgG Type
Human	IgG1/2/3/4
Mouse	IgG1/2a/2b/2c/3
Rat	IgG1/2a/2b/2c
Rabbit	IgG
Dog	IgG

Customers can arbitrarily choose different subtypes for subsequent recombinant expression.

█ IgG Recombinant Antibody Expression Service

TekBiotech is a professional antibody expression customization service company. A full set of Gibco serum-free mammalian cell culture, combined with 293F and CHO suspension cell lines with clear sources, the entire system meets the in vitro recombinant expression needs of different customers for natural conformation proteins. The full set of cost-effective research services provided by TekBiotech to customers meet the GMP-like system, including the cell seed bank source document record system, cell seed batch verification, animal-derived component quality control, standardized cell culture and transformation operation procedures, etc.

Table2 Recombinant IgG antibody expression service content

Steps	Service Content	Period
Step1: Gene cloning and vector construction	-- Amplify/identify gene fragments or synthesize target genes, and insert them into mammalian cell expression plasmids; -- PCR and sequencing tests to determine subcloned genes; -- Delivery: sequencing raw data and experimental report;	1-2 Weeks
Step2: Transient protein expression	-- Recombinant plasmid transfected cells (293F, CHO) transient expression + purification; -- SDS-PAGE and WB detection; endotoxin analysis, ELISA, etc. (optional); -- Delivery: expression vector, cloning strain, recombinant antibody, purity>85%, experimental report;	1-2 Weeks
Step3: Stable expression (optional)	-- Construction and screening of recombinant protein CHO stable expression cell line; -- Delivery: stable cell line, construction report; marginal product, 3 clones, 50ml fermentation product and purified product for each clone;	12–14 Weeks

TekBiotech can also provide customers with one-stop technical services such as antibody humanization, CAR-T/CAR-NK lead sequence design and cell killing verification, to help customers' scientific research projects and drug antibody development.

█ Advantages of Antibody Recombinant Expression Service Platform

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Many service cases, high success rate

Mammalian cell expression platform:

self-designed high expression vector,

optimized independent intellectual

property culture medium and proprietary

transfection reagent

Multiple specifications of amplified

expression system to meet different

customer needs

Guaranteed high-quality service, customized

one-to-one antibody discovery overall solution

Recombinant IgG Customization Services Frequently Asked Questions

What is a recombinant IgG antibody and why choose customization?

Recombinant IgG antibodies are customized antibodies through genetic engineering technology. They are usually composed of two heavy chains and two light chains, completely mimicking IgG antibodies in natural immune responses. Compared with traditional antibody production methods, recombinant IgG antibodies are manufactured by cloning specific antibody gene sequences and expressing them in cells, which provides higher precision and consistency. The advantage of customized recombinant IgG antibodies is that the affinity, specificity and function of antibodies can be customized according to customer needs. Customization services can provide personalized antibody solutions in the early stages of antibody development, especially for antibodies that are difficult to obtain through traditional methods or have specific application requirements. For example, if a customer needs an antibody against a specific antigen, customized recombinant IgG antibodies can ensure high affinity and specificity while avoiding the immune rejection problem in conventional antibody production methods.
How to choose the expression system for recombinant IgG antibody customization services?

When choosing an expression system for recombinant IgG antibodies, the application requirements of the antibody should be considered first. Common expression systems include Escherichia coli, yeast, insect cells, and mammalian cell systems. The Escherichia coli system has low cost and fast expression speed, but it cannot perform complex glycosylation modifications and is suitable for the production of simple antibody fragments or antibodies without glycosylation requirements. Yeast systems can provide limited glycosylation modifications and are suitable for some simple antibody development. Insect cell systems can provide more complex glycosylation modifications and are suitable for expressing larger or complex proteins. For antibodies that require complex glycosylation modifications, especially IgG antibodies prepared for clinical treatment, mammalian cell systems (such as CHO cells, HEK293 cells) are the most commonly used choice because they can simulate the glycosylation pattern in the human body and produce antibodies that are closer to nature. In addition, mammalian systems can also provide higher antibody yields and stability, making them ideal for high-quality antibody production.
What are the advantages of recombinant IgG antibody customization services?

The biggest advantage of recombinant IgG antibody customization services is that they can provide highly specific and high-affinity antibodies, which are essential for clinical research and drug development. Customization services allow customers to design antibodies according to specific needs, whether for specific antigens, pathogens, tumor markers or cell surface receptors, personalized antibodies can be customized. In addition, recombinant IgG antibody customization services can reduce or eliminate possible variation problems in traditional antibody production through genetic engineering technology to ensure product consistency and reliability. In the production process of antibodies, recombinant IgG antibodies have higher flexibility than traditional hybridoma antibodies, and their application effects can be improved by optimizing the affinity and specificity of antibodies. By selecting a suitable expression system (such as CHO cells or HEK293 cells) for antibody production, the stability, glycosylation modification and other properties of antibodies can also be improved to meet the high standards of drug development and biologics.
What are the challenges in the production process of recombinant IgG antibodies?

Although the production process of recombinant IgG antibodies can be optimized by modern biotechnology, it still faces many challenges. First, choosing a suitable expression system is crucial to obtaining high-quality antibodies. Although mammalian cell systems can provide complex glycosylation modifications, they may face problems such as low yield and complex culture conditions during production. Although Escherichia coli expresses rapidly, it cannot perform complex glycosylation modifications, which may lead to incomplete antibody function. In addition, antibody purification is also a technical challenge, especially when the antibody yield is low or high purity is required, multiple rounds of purification steps (such as affinity chromatography, ion exchange chromatography, etc.) are required to remove impurities. On the other hand, the stability of antibodies is also a challenge in production, especially when stored for a long time or produced on a large scale, the folding and activity of antibodies may be affected. Therefore, the expression conditions, purification process and storage scheme need to be carefully optimized during the antibody production process to ensure that the final product has excellent quality and stability.
What are the quality control standards for recombinant IgG antibodies?

The quality control standards for recombinant IgG antibodies usually include multiple aspects to ensure that the antibodies meet the requirements in terms of function, purity, stability, etc. First, affinity and specificity testing are the key to quality control. The binding ability of antibodies to target antigens is evaluated by ELISA, surface plasmon resonance (SPR) and other technologies to ensure that they bind to targets efficiently and have strong affinity. Secondly, purity is the basis for ensuring the quality of antibodies. It is usually tested by SDS-PAGE, HPLC and other technologies to ensure that the impurity content in antibody samples is low and there is no unnecessary degradation or aggregates. Third, stability testing is also important. It is necessary to evaluate the stability of antibodies under different storage conditions, such as long-term stability in low-temperature storage, recovery ability after freeze-thaw, etc. Finally, immunogenicity assessment is crucial for clinical applications, especially for therapeutic antibodies. It is necessary to ensure that the immunogenicity of antibodies is low to avoid adverse reactions to the human immune system.

What is a recombinant IgG antibody and why choose customization?

Recombinant IgG antibodies are customized antibodies through genetic engineering technology. They are usually composed of two heavy chains and two light chains, completely mimicking IgG antibodies in natural immune responses. Compared with traditional antibody production methods, recombinant IgG antibodies are manufactured by cloning specific antibody gene sequences and expressing them in cells, which provides higher precision and consistency. The advantage of customized recombinant IgG antibodies is that the affinity, specificity and function of antibodies can be customized according to customer needs. Customization services can provide personalized antibody solutions in the early stages of antibody development, especially for antibodies that are difficult to obtain through traditional methods or have specific application requirements. For example, if a customer needs an antibody against a specific antigen, customized recombinant IgG antibodies can ensure high affinity and specificity while avoiding the immune rejection problem in conventional antibody production methods.
How to choose the expression system for recombinant IgG antibody customization services?

When choosing an expression system for recombinant IgG antibodies, the application requirements of the antibody should be considered first. Common expression systems include Escherichia coli, yeast, insect cells, and mammalian cell systems. The Escherichia coli system has low cost and fast expression speed, but it cannot perform complex glycosylation modifications and is suitable for the production of simple antibody fragments or antibodies without glycosylation requirements. Yeast systems can provide limited glycosylation modifications and are suitable for some simple antibody development. Insect cell systems can provide more complex glycosylation modifications and are suitable for expressing larger or complex proteins. For antibodies that require complex glycosylation modifications, especially IgG antibodies prepared for clinical treatment, mammalian cell systems (such as CHO cells, HEK293 cells) are the most commonly used choice because they can simulate the glycosylation pattern in the human body and produce antibodies that are closer to nature. In addition, mammalian systems can also provide higher antibody yields and stability, making them ideal for high-quality antibody production.
What are the advantages of recombinant IgG antibody customization services?

The biggest advantage of recombinant IgG antibody customization services is that they can provide highly specific and high-affinity antibodies, which are essential for clinical research and drug development. Customization services allow customers to design antibodies according to specific needs, whether for specific antigens, pathogens, tumor markers or cell surface receptors, personalized antibodies can be customized. In addition, recombinant IgG antibody customization services can reduce or eliminate possible variation problems in traditional antibody production through genetic engineering technology to ensure product consistency and reliability. In the production process of antibodies, recombinant IgG antibodies have higher flexibility than traditional hybridoma antibodies, and their application effects can be improved by optimizing the affinity and specificity of antibodies. By selecting a suitable expression system (such as CHO cells or HEK293 cells) for antibody production, the stability, glycosylation modification and other properties of antibodies can also be improved to meet the high standards of drug development and biologics.
What are the challenges in the production process of recombinant IgG antibodies?

Although the production process of recombinant IgG antibodies can be optimized by modern biotechnology, it still faces many challenges. First, choosing a suitable expression system is crucial to obtaining high-quality antibodies. Although mammalian cell systems can provide complex glycosylation modifications, they may face problems such as low yield and complex culture conditions during production. Although Escherichia coli expresses rapidly, it cannot perform complex glycosylation modifications, which may lead to incomplete antibody function. In addition, antibody purification is also a technical challenge, especially when the antibody yield is low or high purity is required, multiple rounds of purification steps (such as affinity chromatography, ion exchange chromatography, etc.) are required to remove impurities. On the other hand, the stability of antibodies is also a challenge in production, especially when stored for a long time or produced on a large scale, the folding and activity of antibodies may be affected. Therefore, the expression conditions, purification process and storage scheme need to be carefully optimized during the antibody production process to ensure that the final product has excellent quality and stability.
What are the quality control standards for recombinant IgG antibodies?

The quality control standards for recombinant IgG antibodies usually include multiple aspects to ensure that the antibodies meet the requirements in terms of function, purity, stability, etc. First, affinity and specificity testing are the key to quality control. The binding ability of antibodies to target antigens is evaluated by ELISA, surface plasmon resonance (SPR) and other technologies to ensure that they bind to targets efficiently and have strong affinity. Secondly, purity is the basis for ensuring the quality of antibodies. It is usually tested by SDS-PAGE, HPLC and other technologies to ensure that the impurity content in antibody samples is low and there is no unnecessary degradation or aggregates. Third, stability testing is also important. It is necessary to evaluate the stability of antibodies under different storage conditions, such as long-term stability in low-temperature storage, recovery ability after freeze-thaw, etc. Finally, immunogenicity assessment is crucial for clinical applications, especially for therapeutic antibodies. It is necessary to ensure that the immunogenicity of antibodies is low to avoid adverse reactions to the human immune system.

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