Yeast Display Technology Platform-Tekbiotech-Yeast Display Service,Phage display technology

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Yeast Display Technology Platform

TekBiotech is committed to providing customers with high-throughput biological target screening technology solutions. To meet the customized needs of customers, we have developed a library high-throughput screening technology platform based on yeast surface display system of flow cytometry (FACS), which can provide customers with high-throughput screening of BCRs and mTCRs from various species, while meeting the requirements of some customers for affinity pre-grading screening. This technology platform can provide customers with camel-derived VHH nano-antibody development services, scFv discovery services of different species (rabbit, mouse, sheep and camel, etc.), and protein mutant evolution discovery services.

█ Development Services based on Yeast Surface Display Technology

TekBiotech can provide customers with different types of antibody and protein display development services based on yeast surface display technology, scFv antibody development services of different species, and protein mutant evolution screening services. Yeast Display Technology refers to the use of variable regions of antibody sequences to fuse with lectin Aga2p. The Aga2p protein subunit binds to the Aga1p protein subunit fixed on the yeast cell wall through two disulfide bonds. Combined with flow sorting technology, the specific antibodies targeting the antigen are screened out. Based on Yeast Display Technology, TekBiotech can provide customers with high-quality single-chain antibody yeast display services including but not limited to VHH, scFv, etc. The library capacity is up to 10^8, and the library diversity, insertion rate, and positive rate can reach more than 90% to meet the quality requirements of various customers for antibody yeast display libraries. In addition, TekBiotech can also provide antibody drugability evaluation and verification services (including but not limited to affinity verification, antibody blocking verification, cross-reaction verification and other downstream verification experiments), antibody humanization, antibody affinity maturation, CAR-T/CAR-NK lead sequence design and cell killing verification and other one-stop technical services. From antigen preparation to antibody activity verification, the yeast surface display discovery path is shown in Figure 1:

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Figure 1 Antibody discovery based on yeast technology platform

█ Types of Yeast Display Technology Development Services

TekBiotech provides customers with discovery services based on the surface display system of Saccharomyces cerevisiae, as shown in Table 1:

Table 1 Technical services based on yeast surface display technology
Service Type	Species Source
Camel-derived VHH nanoantibody development	Alpaca
Camel-derived VHH nanoantibody development	Camel
scFv antibody development	Human
	Mouse
	Rabbit
mTCRs development service	Human
Protein mutation evolution development	Unlimited (protein cannot be full-length membrane protein)

█ Advantages of Yeast Surface Display Technology Platform Service

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Excellent hydrophobic sequence display, suitable

for TCR surface display

Flexible configuration of single and double

fluorescence screening systems, less antigen

required for screening

Affinity groups can be distinguished during the

screening process

Yeast Display Technology Platform Frequently Asked Questions

What is yeast display technology?

Yeast display technology is a technology that screens, identifies and optimizes proteins or peptides by displaying them on the surface of yeast cells. It uses yeast as a host and relies on the interaction between proteins expressed on the surface of yeast cells and target molecules to achieve efficient molecular discovery and optimization through high-throughput screening and affinity evolution. Yeast display technology is often used in antibody development, enzyme optimization, peptide library screening and vaccine research. Compared with traditional phage display, yeast display technology has higher expression levels, more protein modification capabilities (such as glycosylation modification) and the ability to perform large-scale screening. It is a powerful tool for molecular screening and optimization.
How does yeast display technology work?

Yeast display technology works by fusing the gene for an exogenous protein (e.g., an antibody heavy chain, light chain, or peptide) into the gene for a yeast surface protein, so that the exogenous protein is displayed on the surface of the yeast cell through that protein. The most commonly used display system is the Aga2p fusion system of Saccharomyces cerevisiae (Saccharomyces cerevisiae), in which Aga2p, a lectin on the surface of the yeast, binds to the target protein or peptide via a molecular tag. Exogenous proteins or peptides are synthesized, folded, and surface-displayed by yeast cells, and after binding to target antigens or ligands, molecules with high affinity for the target can be selected by flow-sorting technology, magnetic sorting, or other screening methods.
What are the advantages of yeast display technology over phage display technology?

Yeast display technology has several advantages over phage display technology. First, yeast display technology allows for complex protein modifications on its surface, such as glycosylation, which is important for certain types of antibody and protein functions. Second, the yeast system is more suitable for displaying larger or complex proteins than phages, and the yeast system has a high expression level that enables effective display of target molecules. Third, yeast display technology is easier to perform large-scale cell sorting during the screening process and is able to utilize flow sorting technology to achieve higher screening efficiency. In addition, yeast display is biocompatible and can mimic the biological environment in vivo, providing a more biologically relevant environment for high-affinity antibody or peptide screening.
What are the common application areas of yeast display technology?

Yeast display technology is widely used in antibody discovery, protein engineering, enzyme optimization, vaccine development and peptide library screening. In antibody discovery, yeast demonstration technology can help screen out antibodies with high affinity and specificity to target antigens; in protein engineering, yeast demonstration technology can be used to optimize the stability, affinity, and functional properties of proteins; in enzyme optimization, yeast demonstration technology can be used to obtain enzymes with improved functionality through high-throughput screening to satisfy the needs of the industrial and pharmaceutical fields; moreover, yeast demonstration technology can also be used in the screening of peptide libraries. In addition, yeast display technology can also be used to screen peptide libraries and discover peptide molecules with specific binding ability, which provides the basis for drug design and molecular recognition.
How can yeast display technology optimize antibody affinity?

Yeast display technology can be used in antibody development to optimize the affinity of antibodies through affinity maturation. The specific steps include: first, by constructing an antibody library containing diverse mutations and displaying it on the yeast surface, antibodies with strong affinity for the target antigen are screened. Next, the binding power between the antibody and the antigen is increased by performing further mutations or modifications (e.g., targeted mutations) on the basis of this screening. Antigen-binding antibodies can be separated from other antibodies during the affinity screening process by flow sorting technology or magnetic bead sorting technology. Through multiple rounds of optimization, antibodies with significantly enhanced affinity can be finally obtained for subsequent functional studies and applications.

What is yeast display technology?

Yeast display technology is a technology that screens, identifies and optimizes proteins or peptides by displaying them on the surface of yeast cells. It uses yeast as a host and relies on the interaction between proteins expressed on the surface of yeast cells and target molecules to achieve efficient molecular discovery and optimization through high-throughput screening and affinity evolution. Yeast display technology is often used in antibody development, enzyme optimization, peptide library screening and vaccine research. Compared with traditional phage display, yeast display technology has higher expression levels, more protein modification capabilities (such as glycosylation modification) and the ability to perform large-scale screening. It is a powerful tool for molecular screening and optimization.
How does yeast display technology work?

Yeast display technology works by fusing the gene for an exogenous protein (e.g., an antibody heavy chain, light chain, or peptide) into the gene for a yeast surface protein, so that the exogenous protein is displayed on the surface of the yeast cell through that protein. The most commonly used display system is the Aga2p fusion system of Saccharomyces cerevisiae (Saccharomyces cerevisiae), in which Aga2p, a lectin on the surface of the yeast, binds to the target protein or peptide via a molecular tag. Exogenous proteins or peptides are synthesized, folded, and surface-displayed by yeast cells, and after binding to target antigens or ligands, molecules with high affinity for the target can be selected by flow-sorting technology, magnetic sorting, or other screening methods.
What are the advantages of yeast display technology over phage display technology?

Yeast display technology has several advantages over phage display technology. First, yeast display technology allows for complex protein modifications on its surface, such as glycosylation, which is important for certain types of antibody and protein functions. Second, the yeast system is more suitable for displaying larger or complex proteins than phages, and the yeast system has a high expression level that enables effective display of target molecules. Third, yeast display technology is easier to perform large-scale cell sorting during the screening process and is able to utilize flow sorting technology to achieve higher screening efficiency. In addition, yeast display is biocompatible and can mimic the biological environment in vivo, providing a more biologically relevant environment for high-affinity antibody or peptide screening.
What are the common application areas of yeast display technology?

Yeast display technology is widely used in antibody discovery, protein engineering, enzyme optimization, vaccine development and peptide library screening. In antibody discovery, yeast demonstration technology can help screen out antibodies with high affinity and specificity to target antigens; in protein engineering, yeast demonstration technology can be used to optimize the stability, affinity, and functional properties of proteins; in enzyme optimization, yeast demonstration technology can be used to obtain enzymes with improved functionality through high-throughput screening to satisfy the needs of the industrial and pharmaceutical fields; moreover, yeast demonstration technology can also be used in the screening of peptide libraries. In addition, yeast display technology can also be used to screen peptide libraries and discover peptide molecules with specific binding ability, which provides the basis for drug design and molecular recognition.
How can yeast display technology optimize antibody affinity?

Yeast display technology can be used in antibody development to optimize the affinity of antibodies through affinity maturation. The specific steps include: first, by constructing an antibody library containing diverse mutations and displaying it on the yeast surface, antibodies with strong affinity for the target antigen are screened. Next, the binding power between the antibody and the antigen is increased by performing further mutations or modifications (e.g., targeted mutations) on the basis of this screening. Antigen-binding antibodies can be separated from other antibodies during the affinity screening process by flow sorting technology or magnetic bead sorting technology. Through multiple rounds of optimization, antibodies with significantly enhanced affinity can be finally obtained for subsequent functional studies and applications.

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