Yeast Display Technology Platform-Tekbiotech-Yeast Display Service,Phage display technology

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Yeast Display Technology Platform

TekBiotech is committed to providing customers with high-throughput biological target screening technology solutions. To meet the customized needs of customers, we have developed a library high-throughput screening technology platform based on yeast surface display system of flow cytometry (FACS), which can provide customers with high-throughput screening of BCRs and mTCRs from various species, while meeting the requirements of some customers for affinity pre-grading screening. This technology platform can provide customers with camel-derived VHH nano-antibody development services, scFv discovery services of different species (rabbit, mouse, sheep and camel, etc.), and protein mutant evolution discovery services.

█ Development Services based on Yeast Surface Display Technology

TekBiotech can provide customers with different types of antibody and protein display development services based on yeast surface display technology, scFv antibody development services of different species, and protein mutant evolution screening services. Yeast Display Technology refers to the use of variable regions of antibody sequences to fuse with lectin Aga2p. The Aga2p protein subunit binds to the Aga1p protein subunit fixed on the yeast cell wall through two disulfide bonds. Combined with flow sorting technology, the specific antibodies targeting the antigen are screened out. Based on Yeast Display Technology, TekBiotech can provide customers with high-quality single-chain antibody yeast display services including but not limited to VHH, scFv, etc. The library capacity is up to 10^8, and the library diversity, insertion rate, and positive rate can reach more than 90% to meet the quality requirements of various customers for antibody yeast display libraries. In addition, TekBiotech can also provide antibody drugability evaluation and verification services (including but not limited to affinity verification, antibody blocking verification, cross-reaction verification and other downstream verification experiments), antibody humanization, antibody affinity maturation, CAR-T/CAR-NK lead sequence design and cell killing verification and other one-stop technical services. From antigen preparation to antibody activity verification, the yeast surface display discovery path is shown in Figure 1:

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Figure 1 Antibody discovery based on yeast technology platform

█ Types of Yeast Display Technology Development Services

TekBiotech provides customers with discovery services based on the surface display system of Saccharomyces cerevisiae, as shown in Table 1:

Table 1 Technical services based on yeast surface display technology
Service Type	Species Source
Camel-derived VHH nanoantibody development	Alpaca
Camel-derived VHH nanoantibody development	Camel
scFv antibody development	Human
	Mouse
	Rabbit
mTCRs development service	Human
Protein mutation evolution development	Unlimited (protein cannot be full-length membrane protein)

█ Advantages of Yeast Surface Display Technology Platform Service

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Excellent hydrophobic sequence display, suitable

for TCR surface display

Flexible configuration of single and double

fluorescence screening systems, less antigen

required for screening

Affinity groups can be distinguished during the

screening process

Yeast Display Technology Platform Frequently Asked Questions

Q: Yeast may not be able to effectively express antibodies or have low expression levels, which can affect the production and activity of antibodies.

A: Optimize yeast transformation conditions, select efficient expression vectors, adjust culture conditions (such as temperature, pH, medium composition, etc.), optimize signal peptides and antibody gene sequences, etc., to improve antibody expression levels.
Q: During the construction of yeast display antibody libraries, there may be contamination issues caused by hybridization or non-specific binding of antibodies.

A: Strictly control the construction process of the antibody library, use high-quality antibody genes, and eliminate contaminated antibodies through specific screening and identification to ensure the purity and quality of the library.
Q: There may be a problem of insufficient coverage in the antibody library, that is, not covering all the antigens of interest.

A: By designing a reasonable library construction strategy, including optimizing antigen selection, increasing library capacity and diversity, and using multiple rounds of screening, the coverage of the library can be improved.

Q: Yeast may not be able to effectively express antibodies or have low expression levels, which can affect the production and activity of antibodies.

A: Optimize yeast transformation conditions, select efficient expression vectors, adjust culture conditions (such as temperature, pH, medium composition, etc.), optimize signal peptides and antibody gene sequences, etc., to improve antibody expression levels.
Q: During the construction of yeast display antibody libraries, there may be contamination issues caused by hybridization or non-specific binding of antibodies.

A: Strictly control the construction process of the antibody library, use high-quality antibody genes, and eliminate contaminated antibodies through specific screening and identification to ensure the purity and quality of the library.
Q: There may be a problem of insufficient coverage in the antibody library, that is, not covering all the antigens of interest.

A: By designing a reasonable library construction strategy, including optimizing antigen selection, increasing library capacity and diversity, and using multiple rounds of screening, the coverage of the library can be improved.

Consult Now Yeast Display Technology Platform

Yeast Display Technology Platform

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Yeast Display Technology Platform Frequently Asked Questions

Q: Yeast may not be able to effectively express antibodies or have low expression levels, which can affect the production and activity of antibodies.

Q: During the construction of yeast display antibody libraries, there may be contamination issues caused by hybridization or non-specific binding of antibodies.

Q: There may be a problem of insufficient coverage in the antibody library, that is, not covering all the antigens of interest.

Q: Yeast may not be able to effectively express antibodies or have low expression levels, which can affect the production and activity of antibodies.

Q: During the construction of yeast display antibody libraries, there may be contamination issues caused by hybridization or non-specific binding of antibodies.

Q: There may be a problem of insufficient coverage in the antibody library, that is, not covering all the antigens of interest.

Consult Now Yeast Display Technology Platform