Antibody Druggability Evaluation Service-Tekbiotech-Yeast Display Service,Phage display technology

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Antibody Druggability Evaluation Service

TekBiotech focuses on the development and screening of drug antibodies. After more than 10 years of technology accumulation, it has built a complete yeast surface display technology platform and phage surface display technology platform to screen drug antibody candidates for customers from all over the world.

█ Development Services Based On Yeast Surface Display Technology

TekBiotech can provide customers with different types of antibody and protein display development services based on yeast surface display technology, scFv antibody development services for different species, and protein mutant evolution screening services. Yeast antibody display technology refers to the use of antibody sequence variable region and lectin Aga2p fusion expression, Aga2p protein subunits are bound to Aga1p protein subunits fixed on the yeast cell wall through two disulfide bonds, and combined with flow sorting technology, specific antibodies targeting antigens are screened out. Based on yeast body display technology, TekBiotech can provide customers with high-quality single-chain antibody yeast display services including but not limited to VHH, scFv, etc., with a library capacity of 10^8, and library diversity, insertion rate, and positive rate can reach more than 90%, to meet the quality requirements of various customers for antibody yeast display libraries. In addition, TekBiotech can also provide one-stop technical services such as antibody drugability evaluation and validation services (including but not limited to affinity validation, antibody blocking validation, cross-reaction validation and other downstream validation experiments), antibody humanization, antibody affinity maturation, CAR-T/CAR-NK lead sequence design and cell killing validation. From antigen preparation to antibody activity verification, the yeast surface display discovery path is shown in Figure 1:

Antibody Drugability Evaluation Service-tekbiotech1.png

Figure1 General process of antibody discovery based on yeast technology platform

TekBiotech provides customers with discovery services based on the surface display system of Saccharomyces cerevisiae, as shown in Table 1:

Table1 Technical services based on yeast surface display technology

Service Type	Species Source
Camel-derived VHH nanoantibody development	Alpaca
Camel-derived VHH nanoantibody development	Camel
scFv antibody development	Human
	Mouse
	Rabbit
mTCRs development service	Human
Protein mutation evolution development	Unlimited (protein cannot be full-length membrane protein)

█ Antibody Development Service Based On Phage Display Technology

Based on phage display technology platform technology, Tech Bio can provide customers with camel-derived VHH nanoantibody library, scFv antibody, Fab antibody and other antibody display library construction and screening services from different species. Among them, VHH nanoantibody library, also known as camel-derived heavy chain antibody library, has an antibody molecular weight of 15kDa. It is a unique antibody produced by camel-derived animals and is currently widely used in CAR-T/CAR-NK therapeutic antibody development. scFv and Fab antibodies can come from PBMC cells of hosts such as mice, rats, sheep, rabbits, and humans. From antigen preparation to antibody activity verification, the phage display discovery path is shown in Figure 2:

Antibody Drugability Evaluation Service-tekbiotech2.png

Figure2 General process of antibody discovery based on phage technology platform

TekBiotech provides customers with discovery services based on the surface display system of Saccharomyces cerevisiae, as shown in Table 2:

Table2 Technical services based on yeast surface display technology

Service Type	Species Source
Camel-derived VHH nanoantibody development	Alpaca
Camel-derived VHH nanoantibody development	Camel
scFv antibody development	Human
	Mouse
	Rabbit
mTCRs development service	Human
Protein mutation evolution development	Unlimited (protein cannot be full-length membrane protein)

TekBiotech provides customers with antibody discovery services based on the M13 phage display system, as shown in Table 3:

Table 3 Technical services based on phage surface display technology

Antibody Form	Species Source	Phage Type	Antibody Encapsulation
ScFv antibody form	Human	M13	TG1 library/phage particle library
	Mouse
	Rabbit
	Sheep
Fab form antibody	Human
	Mouse
	Rabbit
VHH nanoantibody	Alpaca
VHH nanoantibody	Camel

█ Antibody Drugability Technology Service

After obtaining various unique antibody sequences by yeast surface display or phage display, TekBiotech uses its own high-throughput electroporation system for transient recombinant expression in the mammalian system and provides a series of antibody drugability evaluation services, such as EC50 detection, FACS verification, etc. For more details, please consult our technical experts.