Issues Related to Aptamer Library Screening-Molecular Biology Service Topics-Tekbiotech-Yeast Display Service,Phage display technology

Issues Related to Aptamer Library Screening

I. Why are more and more studies using DNA libraries instead of RNA libraries in the development of aptamers?

Answer:

1. DNA is more stable: DNA is more stable chemically and biologically, which makes selection and application easier. Especially when using microarray platforms for high-throughput SELEX, the stability of DNA makes the selection process more reliable.

2. DNA libraries are more economical and efficient: Compared with RNA libraries, the selection process of DNA libraries is more time-saving and labor-saving because no additional reverse transcription step is required, which makes DNA libraries more advantageous in terms of time and cost.

3. DNA is easier to synthesize and preserve: From a commercial perspective, DNA is easier to synthesize and is more durable than RNA in terms of shelf life. This makes DNA libraries more advantageous in commercial applications.

II. What are the advantages of G-quadruplex structures in the application of aptamers? How are they associated with specific biological functions?

Answer:

1. Structural stability: G-quadruplex is a higher-order structure formed by a sequence rich in guanine (G). This structure has high stability and can maintain its shape under different environmental conditions, thereby increasing the binding ability of the aptamer to the target.

2. High specificity: The G-quadruplex structure can achieve specific recognition by adjusting the size and sequence of the loop. This structure can interact with specific targets, giving the aptamer a highly specific binding ability.

3. Biological function relevance: Studies have found that the G-quadruplex structure is associated with specific biological functions. For example, certain types of G-quadruplex structures have anti-proliferative activity in cancer cells, but have no effect on non-malignant cells. This means that the G-quadruplex structure can be used to develop aptamers with specific biological functions.

In summary, the application of the G-quadruplex structure in aptamers has the advantages of structural stability, high specificity, and association with specific biological functions. These advantages make the G-quadruplex structure an important tool for designing efficient aptamers.

III. When designing a SELEX library, how to choose the appropriate nucleic acid library type, primer binding site, chemical modification, and randomization strategy to improve the selection effect of the aptamer?

When designing a SELEX library, the following factors need to be considered to improve the selection effect of the aptamer:

1. Selection of nucleic acid library type: Select the appropriate nucleic acid library type according to the properties of the target and the application scenario. For example, you can choose a DNA library or an RNA library, or use genomic encoded RNA or DNA as the library.

2. Selection of primer binding sites: The selection of primer binding sites needs to take into account the structure and characteristics of the target. During the SELEX process, the primer binding site may have an impact on the selection of aptamers. In order to reduce the adverse effects of the primer binding site on the SELEX process, the length of the random region can be optimized and a longer random region can be selected.

3. Application of chemical modification: Chemical modification can increase the stability and specificity of the aptamer. As needed, the aptamer can be chemically modified, such as introducing phosphate modification, phosphate modification or other modifications.

4. Selection of randomization strategy: The randomization strategy can be achieved by different methods. For example, different nucleases or chemical reactions can be used to achieve randomization. Choosing a suitable randomization strategy can increase the diversity and selectivity of the aptamer.

In summary, when designing a SELEX library, factors such as nucleic acid library type, primer binding sites, chemical modification, and randomization strategies need to be considered comprehensively to improve the selection effect of aptamers. The specific selection should be determined according to the properties of the target and the application scenario.

The aptamer in vitro screening service provided by TekBiotech can quickly and accurately screen out aptamers with high affinity and high specificity for customers, saving costs for customers' experimental projects. We flexibly utilize the natural evolution of the ligand system through the exponential enrichment (SELEX) method (for example, the SELEX method can identify different cells or detect differences in the molecular level between two types of cells), providing a one-stop in vitro aptamer screening service.

Previous:Introduction to Nucleic Acid Aptamer Library Screening Technology Next:None!