Molecular Screening and Application of Targeted Peptides-Special Topic on Phage Display Technology-Tekbiotech-Yeast Display Service,Phage display technology

Molecular Screening and Application of Targeted Peptides

Peptides are intermediate products of protein hydrolysis, connected by peptide bonds. Compounds formed by dehydration and condensation of n amino acid molecules are called n-peptides, and tripeptides and above are usually called polypeptides. Methods for peptide synthesis include chemical synthesis and biosynthesis. At present, peptides are widely used in the fields of medicine, beauty, and health care. With more and more problems in medical and health care, the demand for peptide products is increasing. In the field of beauty and health care, it is used as a skin care product in daily life and has the effect of delaying aging. In the medical field, peptides can be used to make vaccines, drugs, etc.

A peptide library composed of a large number of peptides of specific lengths and different sequences, including most of the permutations and combinations of amino acid sequences. According to the source, it is divided into synthetic peptide library, natural peptide library and recombinant protein peptide library. Synthetic peptide library is composed of various peptide sequences prepared by chemical synthesis; natural peptide library is composed of peptides naturally existing in organisms; recombinant protein peptide library is constructed based on protein engineering technology. Methods for peptide library synthesis include phage display technology, mRNA display technology, and one-bead-one-object high-throughput screening technology.

Targeted peptides refer to peptide molecules that can target specific biological targets and have specific affinity. They are used in new targeted therapies and have the advantages of strong penetration, low immunogenicity, and easy chemical synthesis and modification.

1. Progress in Targeted Peptide Screening

There are many methods for screening targeted peptide molecules. Among them, the traditional combinatorial chemical screening method has a long cycle, low efficiency, and time-consuming analysis and identification. The traditional phage display method is difficult to insert or modify non-natural amino acids and has limitations. Therefore, small molecule targeted peptide screening methods have been developed.

① Degeneracy of Peptide Library

The degeneracy of peptide library can increase the probability of target molecules remaining in a small range of peptide library. One degenerate method is to construct a targeted small molecule peptide library. The working principle is to use the specificity of targeted peptides to screen out peptide molecules that interact with the target protein. This technology can be used in the analysis and detection of proteins to develop disease-related diagnostic probes and has a wide range of applications in tumor diagnosis and treatment.

The other is to complete the degeneracy of the peptide library through computer modeling technology. This technology can merge the library capacity of the peptide library, and can predict whether the peptide is closely connected to the molecular target and whether it can inhibit the normal function of the target protein, providing a basis for the rapid screening of drugs.

② High-throughput Screening

Although the degeneracy of the peptide library can improve the efficiency of peptide library screening to a certain extent, it has certain limitations in large-scale screening. Therefore, the development of high-throughput screening technology has gradually developed and is suitable for small molecule targeted peptide screening.

The new mass spectrometry is one of the tools for high-throughput screening. Based on this technology, a high-throughput glycosylated peptide screening method has been developed. This method can greatly improve the throughput of glycopeptide identification and the throughput and accuracy of N-glycoproteomics analysis.

Microfluidic chip systems have also been applied to high-throughput screening technology, with the characteristics of high analysis efficiency, flexible design, automatic control and flux integration.

2. Application of Targeted Peptide Molecules

Peptide drugs are usually peptide analogs synthesized from endogenous peptides. They are one of the hot directions of drug development at present, among which anti-tumor peptides have received much attention. Anti-tumor peptides have anti-cancer effects and are isolated from a variety of natural sources, such as amphibians and reptiles secrete venom with anti-tumor activity, short-tailed hamsters contain can inhibit the proliferation of ovarian cancer and breast cancer.

Through targeted drug screening, a drug that only targets specific cancer cells or proteins can be obtained. When treating cancer diseases, it only harms cancer cells and does not harm normal cells or tissues. Therefore, the use of targeted drugs can improve efficacy and reduce toxic side effects.

TekBiotech has been committed to the field of antibodies for many years and has rich experience and mature technology. It can provide customers with high-quality peptide synthesis, peptide library synthesis and screening technology, targeted drug screening technology, phage display technology, mRNA display technology services, and high-throughput screening technology. In addition, TekBiotech has many years of experience in phage cyclic peptide library screening, and can provide peptide library screening services, phage library screening services, and customized antibody services, including antibody expression and purification, affinity determination, antibody sequencing, etc., to meet customer needs.

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