DNA Immune Antibody Preparation Service-Tekbiotech-Yeast Display Service,Phage display technology

Services

Inquiry and ordering

Name*
Phone*
Email*
Product*
Content*

DNA Immune Antibody Preparation Service

As an advanced technical method, DNA immunization can effectively induce antibody and T cell immune responses against various pathogens, and effectively induce high-quality polyclonal antibody responses in immunized animals or human serum, with high conformational specificity and high affinity. Unlike conventional immunization methods, DNA immunization enables the host to produce target antigens with common post-transcriptional modifications in vivo, and the antigen maintains the original structure to a large extent, bypassing the time-consuming synthesis and purification process of immunogens (such as peptides and recombinant proteins). DNA immunization characteristics help to produce high-affinity antibodies against the natural conformation of the target antigen, which is also a key feature for the development of therapeutic antibodies. At the same time, this method is also an ideal solution for the development of antibodies against membrane proteins or other challenging antigens. In addition, DNA immunization is also a new tool in the early stages of immunotherapy and DNA vaccine development.

DNA Immune Antibody Preparation Service-tekbiotech1.png

Figure1 AAV virus particles

Tekbiotech can provide customers with DNA immune antibody preparation services for a variety of antigens (including but not limited to PTM proteins, intracellular proteins, secretory proteins), antibody drug preparation service platforms based on hybridoma fusion and screening technology, and Tekbiotech can provide customers with a series of services related to the preparation of therapeutic biomacromolecule immune antibodies, including but not limited to VHH nanoantibodies, scFv antibodies, Fab antibodies, DNA immune antibodies and other preparation services. After 10 years of development, Tekbiotech has established a complete hybridoma fusion and screening platform, and has the ability to provide customers with a series of high-quality DNA immune antibody preparation services based on hybridoma fusion and screening technology platforms. In addition, Tekbiotech has one-stop technical services including gene synthesis and modification, AAV vector construction, animal immunization, hybridoma cell fusion and screening, antibody production, etc., and has supporting antigen-antibody binding analysis, antigen-antibody BLI affinity determination, antibody cell line verification, and many other downstream supporting services, providing strong support for customers' antibody drug research and development and production.

█ DNA Immunization Delivery Methods

Common DNA immunization methods include gene gun, intramuscular injection, and subcutaneous direct injection of DNA plasmids. The above methods have high requirements for laboratory instruments and equipment. TekBiotech has developed a set of mature DNA immunization methods based on AAV virus delivery methods, which can ensure the provision of high-quality animal immunization services to customers, reduce the demand for protein and cell-based immune antigens, and thus save customers time and costs.

Delivery Method	Targeted Antigen Type
Gene Gun	Single transmembrane protein
	Intracellular protein
	Viral coat protein
Muscular Injection	Bacterial toxin
	7-transmembrane protein
	Viral coat protein
	Secreted protein, enzyme
Intradermal Injection	Single transmembrane protein
	Extracellular matrix and plasma
	Glycoprotei

█ DNA Immunization Service Process (Taking Virus as Vector, Alpaca Nanoantibody Development as an Example)

(Note: Customers only need to provide a very small amount of VLP protein or target antigen expression cell line to verify the DNA immunization titer)

Service Items	Content	Cycle
Step 1: DNA virus synthesis	1) Gene template synthesis, codon optimization, related AAV vector construction 2) Virus packaging + QC control	4-6 Weeks
Step2: Animal immunization	1) Multiple immunization of alpaca 2) ELISA serum titer detection	8-10 Weeks
Step3: Antibody screening	1) Alpaca blood collection and PBMC isolation 2) Phage library or yeast antibody display library construction 3) Screening to obtain VHH antibody sequence	4-6 Weeks
Step 4: Antibody production	1) Antibody recombinant expression 2) Antibody activity verification	3-4 Weeks

█ DNA Immunization Advantages

-- Compared with DNA vectors, mRNA is easier to obtain, high quality and cheap

-- No need for recombinant protein, worry-free full-length membrane protein immunization, low cost (compared to VLP)

-- No need to purify VLP protein, suitable for ion channel protein and multi-transmembrane protein antibody development

-- Fewer immunization times, high immune titer

█ Service Advantages

DNA Immune Antibody Preparation Service-tekbiotech2.png

DNA Immune Antibody Preparation Service-tekbiotech3.png

DNA Immune Antibody Preparation Service-tekbiotech4.png

DNA Immune Antibody Preparation Service-tekbiotech5.png

Customized immunization program, high

specificity of membrane protein target

immunity, high purity of mRN immune

antigen (compared with cell immunity)

Rich experience in immunization, guaranteed

animal immune titer

Suitable for ion channel protein, multiple

transmembrane protein antibody development,

many cases

Provide detailed immunization schedule and

immunization program

DNA Immune Antibody Preparation Service Frequently Asked Questions

What is DNA immunization antibody preparation service? What are its main applications?

DNA immunization antibody preparation service is an antibody production technology based on DNA vaccines. By injecting the DNA sequence encoding the target antigen into the animal, the cells in the animal are induced to synthesize the antigen and stimulate the immune system to produce specific antibodies. This technology is different from the traditional protein immunization method because it uses DNA as an expression vector rather than the antigen itself. DNA immunization can quickly and effectively generate antibodies against a variety of targets and is widely used in vaccine development, antibody drug development, tumor immunotherapy and disease marker screening. It is particularly suitable for rapid response to emerging disease pathogens and has the advantages of high efficiency, flexibility and relatively low cost.
What are the advantages of DNA immunization antibody preparation over traditional protein immunization methods?

DNA immunization antibody preparation has several obvious advantages over traditional protein immunization methods. First, DNA immunization does not require the synthesis of a large amount of antigen protein in advance, which greatly reduces production costs and time. Especially when it is necessary to respond quickly to emerging pathogens, DNA immunization can significantly shorten the antibody development cycle. Secondly, DNA immunization avoids the folding problem that may occur during the in vitro synthesis of antigen proteins by directly expressing antigens in host cells, and can produce a more natural immune response. In addition, DNA immunization technology is highly flexible and can be quickly optimized for different antigens by adjusting the design of DNA sequences, which is suitable for immune development of multiple antigen types. Finally, DNA immunization usually induces a wider range of immune responses, including antibody-mediated immune responses and T cell-mediated immune responses, increasing multi-level immune protection.
What are the advantages of DNA immunization antibody preparation services?

DNA immunization antibody preparation services have multiple advantages. First, DNA immunization provides a simple and low-cost antibody development approach, eliminating the time and cost required for traditional protein expression. Secondly, since DNA immunization is based on gene expression, antigens do not require a large number of purification steps when expressed in host cells, reducing the complexity and cost of the production process. In addition, DNA immunization can trigger a more comprehensive immune response, which can not only activate B cells to produce antibodies, but also promote T cell immune responses, thereby providing stronger immune protection. Since DNA can be continuously expressed in animals, the immune response generated is often more persistent. In addition, DNA immunization can flexibly adjust antigen design to adapt to the immune needs of a variety of different targets, and has high application value.
How to evaluate the affinity and specificity of antibodies produced by DNA immunization?

The evaluation of the affinity and specificity of antibodies produced by DNA immunization is usually achieved through in vitro immunological methods. The most common method is ELISA (enzyme-linked immunosorbent assay), which can evaluate the affinity of antibodies by detecting the binding reaction between antibodies and target antigens. Usually, in ELISA, different concentrations of antigens can be used to draw the binding curve of antibodies to calculate their affinity constant (Kd). In addition, the specificity of antibodies can be verified by methods such as Western blot and immunoprecipitation. In order to further verify the functionality of antibodies, flow cytometry, immunohistochemistry and other techniques can also be used to evaluate the specific recognition ability of antibodies in cells and tissues. Through these experiments, high-quality antibodies with high affinity and low cross-reaction can be screened.
What are the advantages of DNA immunization antibody preparation services in drug development?

DNA immunization antibody preparation services have significant advantages in drug development. First, DNA immunization can quickly generate high-affinity antibodies, especially in the development of new diseases or targets, which can provide rapid response for drug development. Secondly, DNA immunization can directly produce target antigens in vivo, and the immune response is closer to the natural physiological state, which can improve the biological activity and stability of antibodies. In addition, DNA immunization significantly reduces production costs by reducing the need for protein production and purification, while having high scalability and flexibility, and can customize antibodies according to different needs. Antibodies obtained through DNA immunization can effectively evaluate their potential therapeutic effects in preclinical studies, especially in drug development in the fields of autoimmune diseases, infectious diseases and cancer, which can provide more options and opportunities.

What is DNA immunization antibody preparation service? What are its main applications?

DNA immunization antibody preparation service is an antibody production technology based on DNA vaccines. By injecting the DNA sequence encoding the target antigen into the animal, the cells in the animal are induced to synthesize the antigen and stimulate the immune system to produce specific antibodies. This technology is different from the traditional protein immunization method because it uses DNA as an expression vector rather than the antigen itself. DNA immunization can quickly and effectively generate antibodies against a variety of targets and is widely used in vaccine development, antibody drug development, tumor immunotherapy and disease marker screening. It is particularly suitable for rapid response to emerging disease pathogens and has the advantages of high efficiency, flexibility and relatively low cost.
What are the advantages of DNA immunization antibody preparation over traditional protein immunization methods?

DNA immunization antibody preparation has several obvious advantages over traditional protein immunization methods. First, DNA immunization does not require the synthesis of a large amount of antigen protein in advance, which greatly reduces production costs and time. Especially when it is necessary to respond quickly to emerging pathogens, DNA immunization can significantly shorten the antibody development cycle. Secondly, DNA immunization avoids the folding problem that may occur during the in vitro synthesis of antigen proteins by directly expressing antigens in host cells, and can produce a more natural immune response. In addition, DNA immunization technology is highly flexible and can be quickly optimized for different antigens by adjusting the design of DNA sequences, which is suitable for immune development of multiple antigen types. Finally, DNA immunization usually induces a wider range of immune responses, including antibody-mediated immune responses and T cell-mediated immune responses, increasing multi-level immune protection.
What are the advantages of DNA immunization antibody preparation services?

DNA immunization antibody preparation services have multiple advantages. First, DNA immunization provides a simple and low-cost antibody development approach, eliminating the time and cost required for traditional protein expression. Secondly, since DNA immunization is based on gene expression, antigens do not require a large number of purification steps when expressed in host cells, reducing the complexity and cost of the production process. In addition, DNA immunization can trigger a more comprehensive immune response, which can not only activate B cells to produce antibodies, but also promote T cell immune responses, thereby providing stronger immune protection. Since DNA can be continuously expressed in animals, the immune response generated is often more persistent. In addition, DNA immunization can flexibly adjust antigen design to adapt to the immune needs of a variety of different targets, and has high application value.
How to evaluate the affinity and specificity of antibodies produced by DNA immunization?

The evaluation of the affinity and specificity of antibodies produced by DNA immunization is usually achieved through in vitro immunological methods. The most common method is ELISA (enzyme-linked immunosorbent assay), which can evaluate the affinity of antibodies by detecting the binding reaction between antibodies and target antigens. Usually, in ELISA, different concentrations of antigens can be used to draw the binding curve of antibodies to calculate their affinity constant (Kd). In addition, the specificity of antibodies can be verified by methods such as Western blot and immunoprecipitation. In order to further verify the functionality of antibodies, flow cytometry, immunohistochemistry and other techniques can also be used to evaluate the specific recognition ability of antibodies in cells and tissues. Through these experiments, high-quality antibodies with high affinity and low cross-reaction can be screened.
What are the advantages of DNA immunization antibody preparation services in drug development?

DNA immunization antibody preparation services have significant advantages in drug development. First, DNA immunization can quickly generate high-affinity antibodies, especially in the development of new diseases or targets, which can provide rapid response for drug development. Secondly, DNA immunization can directly produce target antigens in vivo, and the immune response is closer to the natural physiological state, which can improve the biological activity and stability of antibodies. In addition, DNA immunization significantly reduces production costs by reducing the need for protein production and purification, while having high scalability and flexibility, and can customize antibodies according to different needs. Antibodies obtained through DNA immunization can effectively evaluate their potential therapeutic effects in preclinical studies, especially in drug development in the fields of autoimmune diseases, infectious diseases and cancer, which can provide more options and opportunities.

Consult Now DNA Immune Antibody Preparation Service

Animal Immunization Technology Services

Recommended Services

DNA Immune Antibody Preparation Service Frequently Asked Questions

What is DNA immunization antibody preparation service? What are its main applications?

What are the advantages of DNA immunization antibody preparation over traditional protein immunization methods?

What are the advantages of DNA immunization antibody preparation services?