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mRNA Immune Antibody Preparation Service

In recent years, with the development of mRNA synthesis, chemical modification of mRNA and delivery technology, the stability and translation efficiency of mRNA have been greatly improved, and the immunogenicity has gradually become controllable. It is widely used in the field of tumor immunotherapy and vaccines. After years of animal immunization, TekBiotech has formed a unique set of nucleic acid immunization technologies, including mRNA immunization and DNA immunization. Among them, mRNA immunization technology has become mature and is suitable for the development of neutralizing blocking antibodies for GPCR receptor proteins, ion channel proteins and complex receptor proteins (such as CD3 complex protein blocking antibodies).

mRNA Immune Antibody Preparation Service-tekbiotech1.png

Figure1 mRNA LNP encapsulated particles

TekBiotech can provide customers with mRNA immune antibody preparation services for a variety of antigens (including but not limited to PTM proteins, intracellular proteins, secreted proteins). In addition, TekBiotech has one-stop technical services including gene synthesis and modification, AAV vector construction, animal immunization, hybridoma cell fusion and screening, antibody production, etc., and has supporting antigen-antibody binding analysis, antigen-antibody BLI affinity determination, antibody cell line verification, and many other downstream supporting services, providing strong support for customers' antibody drug research and development and production.

█ mRNA Immunization Service Process (Taking Nano-antibodies as an Example)

Service Items	Content	Cycle
Step 1: mRNA synthesis	1) mRNA synthesis and packaging 2) QC control	2-3 Weeks
Step 2: Animal immunization	1) Multiple immunization of alpacas 2) ELISA serum titer detection	8-10 Weeks
Step 3: Antibody screening	1) Alpaca blood collection and PBMC isolation 2) Phage library or yeast antibody display library construction 3) Screening to obtain VHH antibody sequence	4-6 Weeks
Step 4: Antibody production	1) Antibody recombinant expression 2) Antibody activity verification	3-4 Weeks

█ mRNA Immune Advantages

-- Easy to obtain in vitro, high purity, cost saving

-- No need for recombinant protein, worry-free full-length membrane protein immunization, low cost (compared to WLP)

-- No need to purify VLP protein, suitable for ion channel protein and multiple transmembrane protein antibody development

-- Fewer immunization times, high immune titer

█ Service Advantages

Rich experience in immunization, guaranteed

animal immunization titer

Suitable for the development of ion channel

proteins and multiple transmembrane protein

antibodies, with many cases

Provide detailed immunization schedule

and immunization plan

Customized immunization plan, high

specificity of membrane protein targets,

high purity of mRNA immune antigens

(compared to cell-based immunization)

mRNA Immune Antibody Preparation Service Frequently Asked Questions

What is the mRNA immune antibody preparation service? What are its main applications?

The mRNA immune antibody preparation service is an innovative method to achieve antibody immunity through mRNA technology. By introducing mRNA encoding the target antigen into the host cell, it is expressed and displayed in the body, thereby stimulating the immune system to produce antibodies. This service is often used in areas such as antibody development, vaccine research, and antibody drug discovery. Compared with traditional protein immunization methods, mRNA immunization has higher flexibility and scalability, and can quickly respond to the needs of new targets. In terms of application, mRNA immunization can be used to quickly generate antibodies against specific pathogens or tumors, and is widely used in antibody development in infectious diseases, cancer immunotherapy, and allergy immunity.
What is the basic principle of mRNA immune antibody preparation?

The basic principle of mRNA immune antibody preparation is to use mRNA as a gene expression vector to deliver mRNA encoding the target antigen to host cells (such as in vivo or in vitro cells of mice, rabbits, or other experimental animals). After receiving the mRNA, the host cell uses its inherent translation mechanism to synthesize the target antigen. After the immune system recognizes these synthesized antigen molecules, it will produce specific antibodies. By regularly sampling and analyzing the immune response in animals, antibodies with high affinity and specificity for the target antigen can be screened. mRNA immunization provides a more efficient and flexible way to produce antibodies by simulating the natural immune process of pathogen infection.
How does mRNA immunization antibody preparation service differ from traditional antibody preparation methods?

mRNA immunization antibody preparation service has multiple advantages over traditional antibody preparation methods (such as protein immunization and cell immunization). First, mRNA immunization does not require cloning and expression of antigen proteins, avoiding the common difficulties and costs in protein production. Second, mRNA immunization can directly guide the production of target antigens in animals, and the immune response is closer to the natural physiological state, so it may produce antibodies with higher affinity and specificity. In addition, mRNA immunization is more time-efficient, and preliminary immune response results can usually be obtained within a few weeks, while traditional methods may take months. In addition, mRNA immunization is more flexible and scalable, which is particularly important for rapid response to emerging diseases or antibody development for undiscovered targets.
What are the advantages of mRNA immunization antibody preparation?

The advantages of mRNA immunization antibody preparation are mainly reflected in its efficiency, flexibility and cost-effectiveness. First, mRNA immunization can quickly respond to different targets and is particularly suitable for antibody development for emerging diseases. Since there is no need to produce large amounts of protein or cell culture, this method has lower production costs and shorter development cycles. Second, mRNA technology can efficiently regulate immune responses and can induce antibodies with higher affinity. Compared with traditional immunization methods, mRNA immunization can avoid the complexity of protein expression and can directly synthesize antigens in host cells, reducing possible errors in the production process. Finally, the mRNA immunization method is highly customizable and can adjust the immunization strategy according to different needs (such as antigen type, immune strength, etc.), and has strong scalability.
How to evaluate the affinity and specificity of antibodies produced by mRNA immunization?

The evaluation of the affinity and specificity of antibodies produced by mRNA immunization is mainly done through in vitro experiments. First, ELISA (enzyme-linked immunosorbent assay) can be used to detect whether the antibody can specifically bind to the target antigen, and the affinity can be evaluated by measuring the half-maximal inhibitory concentration (IC50). Second, the binding kinetics between the antibody and the target cell or target antigen can be analyzed by flow cytometry or surface plasmon resonance (SPR) to further confirm its affinity and binding constant. For specificity assessment, in addition to specific binding to the target, it is also necessary to ensure that the antibody does not produce cross-reactions by testing other non-target molecules. In addition, functional experiments, such as neutralization experiments, can be used to detect the biological activity of antibodies in actual applications.

What is the mRNA immune antibody preparation service? What are its main applications?

The mRNA immune antibody preparation service is an innovative method to achieve antibody immunity through mRNA technology. By introducing mRNA encoding the target antigen into the host cell, it is expressed and displayed in the body, thereby stimulating the immune system to produce antibodies. This service is often used in areas such as antibody development, vaccine research, and antibody drug discovery. Compared with traditional protein immunization methods, mRNA immunization has higher flexibility and scalability, and can quickly respond to the needs of new targets. In terms of application, mRNA immunization can be used to quickly generate antibodies against specific pathogens or tumors, and is widely used in antibody development in infectious diseases, cancer immunotherapy, and allergy immunity.
What is the basic principle of mRNA immune antibody preparation?

The basic principle of mRNA immune antibody preparation is to use mRNA as a gene expression vector to deliver mRNA encoding the target antigen to host cells (such as in vivo or in vitro cells of mice, rabbits, or other experimental animals). After receiving the mRNA, the host cell uses its inherent translation mechanism to synthesize the target antigen. After the immune system recognizes these synthesized antigen molecules, it will produce specific antibodies. By regularly sampling and analyzing the immune response in animals, antibodies with high affinity and specificity for the target antigen can be screened. mRNA immunization provides a more efficient and flexible way to produce antibodies by simulating the natural immune process of pathogen infection.
How does mRNA immunization antibody preparation service differ from traditional antibody preparation methods?

mRNA immunization antibody preparation service has multiple advantages over traditional antibody preparation methods (such as protein immunization and cell immunization). First, mRNA immunization does not require cloning and expression of antigen proteins, avoiding the common difficulties and costs in protein production. Second, mRNA immunization can directly guide the production of target antigens in animals, and the immune response is closer to the natural physiological state, so it may produce antibodies with higher affinity and specificity. In addition, mRNA immunization is more time-efficient, and preliminary immune response results can usually be obtained within a few weeks, while traditional methods may take months. In addition, mRNA immunization is more flexible and scalable, which is particularly important for rapid response to emerging diseases or antibody development for undiscovered targets.
What are the advantages of mRNA immunization antibody preparation?

The advantages of mRNA immunization antibody preparation are mainly reflected in its efficiency, flexibility and cost-effectiveness. First, mRNA immunization can quickly respond to different targets and is particularly suitable for antibody development for emerging diseases. Since there is no need to produce large amounts of protein or cell culture, this method has lower production costs and shorter development cycles. Second, mRNA technology can efficiently regulate immune responses and can induce antibodies with higher affinity. Compared with traditional immunization methods, mRNA immunization can avoid the complexity of protein expression and can directly synthesize antigens in host cells, reducing possible errors in the production process. Finally, the mRNA immunization method is highly customizable and can adjust the immunization strategy according to different needs (such as antigen type, immune strength, etc.), and has strong scalability.
How to evaluate the affinity and specificity of antibodies produced by mRNA immunization?

The evaluation of the affinity and specificity of antibodies produced by mRNA immunization is mainly done through in vitro experiments. First, ELISA (enzyme-linked immunosorbent assay) can be used to detect whether the antibody can specifically bind to the target antigen, and the affinity can be evaluated by measuring the half-maximal inhibitory concentration (IC50). Second, the binding kinetics between the antibody and the target cell or target antigen can be analyzed by flow cytometry or surface plasmon resonance (SPR) to further confirm its affinity and binding constant. For specificity assessment, in addition to specific binding to the target, it is also necessary to ensure that the antibody does not produce cross-reactions by testing other non-target molecules. In addition, functional experiments, such as neutralization experiments, can be used to detect the biological activity of antibodies in actual applications.

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mRNA Immune Antibody Preparation Service Frequently Asked Questions

What is the mRNA immune antibody preparation service? What are its main applications?

What is the basic principle of mRNA immune antibody preparation?

How does mRNA immunization antibody preparation service differ from traditional antibody preparation methods?