Peptide Affinity Maturation Services-Tekbiotech-Yeast and Phage Display CRO, Expert in Nano-body and Antibody Drug Development

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Peptide Affinity Maturation Services

Homing peptide (i.e., targeted peptide) have become highly promising targeted small-molecule drug candidates due to their small molecular weight, good tissue permeability, ease of synthesis, modification, and customization, low immunogenicity, and low production costs, homing peptides have emerged as highly promising small-molecule drug candidates for targeted therapy. They are also a major focus of research in the treatment of diseases involving “hard-to-drug” targets (such as p53-MDM2 and other PPI targets) and in oncology (such as tumor-associated targets like PD-L1 and EGFR). However, the development of homing peptides and related drugs (such as peptide-drug conjugates (PDCs) and radiopharmaceuticals) requires them to possess very high affinity to achieve effective targeted diagnostic and therapeutic outcomes. Therefore, affinity maturation has become an indispensable step in the development of homing peptide drugs. TekBiotech specializes in providing clients with high-quality homing peptide discovery and early-stage development services for related drugs (e.g., therapeutic PDCs, diagnostic-therapeutic nuclear pharmaceuticals, etc.). Through years of technical expertise, the company has established a set of efficient peptide affinity maturation strategies suitable for downstream homing peptide drug development.

█ Peptide Affinity Maturation Services

PDC drugs used for therapy and radiopharmaceuticals used for diagnostic and therapeutic integration generally require homing peptides with high affinity. Therefore, when the affinity of a homing peptide fails to meet the requirements of late-stage drug development, it must be optimized through affinity maturation to obtain a homing peptide sequence with higher affinity. Enhancing the affinity of homing peptides requires proven and effective solutions. With over a decade of technical expertise, TekBiotech has established a highly efficient peptide affinity maturation strategy to assist clients with professional analysis of the parent homing peptide sequence (i.e., analysis of the target protein and peptide binding sites) and the design of mutation sites. Leveraging mature phage display and yeast display platforms, TekBiotech maximizes the affinity of the parent homing peptide.

Based on affinity data for the parent homing peptide sequence, TekBiotech synthesizes analyzed and designed mutation gene libraries using methods such as Trimer Codon, NNK, or ePCR. Leveraging phage display and yeast display systems, the company constructs high-quality peptide sequence mutation libraries and identifies candidate targeted peptide sequences that meet the target requirements through effective screening methods. TekBiotech’s experts develop customized peptide affinity maturation strategies based on target information provided by clients to ensure the success rate of client projects and the effectiveness of downstream drug development.

Figure 1- Peptide Affinity Maturation Services Based on a Surface Display Technology Platform.png

Figure 1: Peptide Affinity Maturation Services Based on a Surface Display Technology Platform

█ Details and Timeline for Peptide Affinity Maturation Services

Steps	Service Details	Weeks
Step 1: Construction of Phage Display Library	(1) Analysis of the target protein–peptide binding site to identify mutation sites; (2) Synthesis of a mutant gene library using the Trimer Condon technology; (3) Construction of phage display library;	5-7 weeks
Step 2: Screening of Phage Display Libraries	(1) Phage display library screening: 3 rounds of screening; (2) Random selection of single clone from the products of the 3 rounds of screening + sequencing; (3) Random selection of 5 peptides for synthesis + affinity detection;	3-4 weeks
Step 3: Construction of Yeast Display Library	(1) Gene amplification of products from the three rounds of screening in Step 2; (2) Construction of yeast display library;	3-4 weeks
Step 4: Screening of the Yeast Display Library	(1) Screening of the yeast display library: 3 rounds of screening (FACS screening); (2) NGS sequencing and sequence analysis of the products from the 3 rounds of screening; (3) Synthesis of 1–2 peptides and detection of their affinity;	3-4 weeks

TekBiotech also provides clients with downstream homing peptide drug development and preliminary drugability evaluation services, such as PDC development, design and optimization of preclinical development strategies for radiopharmaceuticals, affinity detection, cell binding detection, cytotoxicity detection, and animal model detection—a one-stop technical service designed to support clients’ research projects and homing peptide drug development.

█ Advantages of Peptide Affinity Maturation Services


A Mature Platform for Downstream Development of Homing Peptide drug Drugs and Preliminary Drug Candidate Evaluation	A Mature Affinity Enhancement Platform Capable of Achieving Affinities in the nM Range	Customized Analysis and Design of Affinity Maturity Sites	Guaranteed, High-Quality Service