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mRNA Antibody Production Services

With continued breakthroughs in mRNA design, synthesis, and delivery technologies, mRNA stability and translation efficiency have improved significantly, and immunogenicity can now be precisely regulated. As a result, its applications are becoming increasingly widespread in fields such as cancer immunotherapy, vaccine development, and the development of targeted functional therapeutic antibodies.

TekBiotech has been deeply involved in mRNA immunization technology for many years and has established a mature system for mRNA design, synthesis, LNP encapsulation, and animal immunization. The company is capable of designing appropriate mRNA antigens and LNP formulations based on the characteristics of the client’s targets and desired antibodies, while implementing rigorous quality control. The resulting mRNA-LNP products feature complete target sequences, high purity, high encapsulation efficiency, high particle uniformity, and high immunogenicity. making them suitable for downstream immunological applications. Combined with TekBiotech’s diversified immunization protocol design—which employs multiple immunization strategies in parallel—the resulting animal immunization sera exhibit high titers and high antibody diversity, facilitating the downstream screening of various functional targeted monoclonal antibodies (e.g., blocking antibodies, non-blocking targeted antibodies, and antibodies targeting specific functional epitopes). TekBiotech possesses unique insights and extensive experience in the development of antibodies against various multi-transmembrane proteins, with particular expertise in the development of neutralizing and blocking antibodies against GPCR receptor proteins, ion channel proteins, and receptor proteins in complex forms (such as blocking antibodies against complex proteins like TCR, CD3, and FcRn).

Leveraging its extensive technical expertise, TekBiotech has launched nearly 200 types of off-the-shelf mRNA plasmids, with a focus on CAR-T cell therapy, ADC development, the development of blocking targeted antibody drugs, and the precise design of tumor-targeted genes. The company can complete mRNA packaging and shipping within 7 days, providing customers with efficient and convenient raw material support. Additionally, it offers professional mRNA preparation and immunological technical services for a variety of antigens (including PTM proteins, intracellular proteins, and secreted proteins), as well as downstream functional antibody development services.

TekBiotech not only provides end-to-end services ranging from mRNA immunogen design and synthesis, through animal immunization, to antibody screening (phage display/yeast display systems), but also possesses extensive experience in transmembrane protein immunization, offering standalone animal immunization services and one-on-one customized immunization protocol design. Combined with a comprehensive downstream antibody functional validation platform, TekBiotech helps you rapidly obtain high-affinity antibodies targeting complex membrane proteins, thereby accelerating the drug discovery process.

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Figure 1: mRNA LNP-encapsulated particles

In addition, TekBiotech has a well-established downstream antibody drug candidate evaluation system, offering a range of supporting validation services such as antigen-antibody binding assays (including affinity validation and competitive binding assays), in vitro efficacy evaluations (including flow cytometry-based blocking assays, cell-binding activity analysis, epitope binding analysis, and cytotoxic activity validation), and in vivo efficacy evaluations (animal model experiments and in vivo imaging). TekBiotech can also carry out subsequent antibody product development, such as CAR-T development, ADC development, and bispecific antibody development, providing robust support for clients’ antibody drug R&D and production.

█ mRNA Immunization Service Procedure

Figure 2 mRNA Immunization Service Procedure

█ mRNA Immunology Services (Using Nanobodies as an Example)

Service Steps	Services Details	Weeks
Step 1: mRNA Synthesis	(1) mRNA synthesis and packaging; (2) QC control;	1-2 weeks
Step 2: Animal Immunization	(1) Immunizations of alpacas; (2) ELISA serum titer testing;	8-10 weeks
Step 3: Antibody Screening	(1) Collect blood from alpacas and isolate PBMCs; (2) Construct a phage library or a yeast antibody display library; (3) Screen the library to obtain VHH antibody sequences;	4-6 weeks
Step 4: Antibody Production	(1) Recombinant antibody expression; (2) Detection of antibody activity;	3-4 weeks

█ Advantages of mRNA Immunological Services

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Specializing in the Development of Antibodies Against Membrane Proteins

Target functional antibodies are efficiently obtained through direct immunization with mRNA.

Diversified Immunization Strategies

Combining multiple immunization methods can achieve higher titers and greater antibody diversity.

“One-Stop” Delivery Guarantee

One-stop services and comprehensive solutions, covering antigen design and production, all the way to the delivery of active antibodies.

Customized Immunization Programs

Customized plan, along with close monitoring of antibody titers, is implemented to ensure the achievement of the expected immune effect.

mRNA Antibody Production Services Frequently Asked Questions

What is the mRNA immune antibody preparation service? What are its main applications?

The mRNA immune antibody preparation service is an innovative method to achieve antibody immunity through mRNA technology. By introducing mRNA encoding the target antigen into the host cell, it is expressed and displayed in the body, thereby stimulating the immune system to produce antibodies. This service is often used in areas such as antibody development, vaccine research, and antibody drug discovery. Compared with traditional protein immunization methods, mRNA immunization has higher flexibility and scalability, and can quickly respond to the needs of new targets. In terms of application, mRNA immunization can be used to quickly generate antibodies against specific pathogens or tumors, and is widely used in antibody development in infectious diseases, cancer immunotherapy, and allergy immunity.
What is the basic principle of mRNA immune antibody preparation?

The basic principle of mRNA immune antibody preparation is to use mRNA as a gene expression vector to deliver mRNA encoding the target antigen to host cells (such as in vivo or in vitro cells of mice, rabbits, or other experimental animals). After receiving the mRNA, the host cell uses its inherent translation mechanism to synthesize the target antigen. After the immune system recognizes these synthesized antigen molecules, it will produce specific antibodies. By regularly sampling and analyzing the immune response in animals, antibodies with high affinity and specificity for the target antigen can be screened. mRNA immunization provides a more efficient and flexible way to produce antibodies by simulating the natural immune process of pathogen infection.
How does mRNA immunization antibody preparation service differ from traditional antibody preparation methods?

mRNA immunization antibody preparation service has multiple advantages over traditional antibody preparation methods (such as protein immunization and cell immunization). First, mRNA immunization does not require cloning and expression of antigen proteins, avoiding the common difficulties and costs in protein production. Second, mRNA immunization can directly guide the production of target antigens in animals, and the immune response is closer to the natural physiological state, so it may produce antibodies with higher affinity and specificity. In addition, mRNA immunization is more time-efficient, and preliminary immune response results can usually be obtained within a few weeks, while traditional methods may take months. In addition, mRNA immunization is more flexible and scalable, which is particularly important for rapid response to emerging diseases or antibody development for undiscovered targets.
What are the advantages of mRNA immunization antibody preparation?

The advantages of mRNA immunization antibody preparation are mainly reflected in its efficiency, flexibility and cost-effectiveness. First, mRNA immunization can quickly respond to different targets and is particularly suitable for antibody development for emerging diseases. Since there is no need to produce large amounts of protein or cell culture, this method has lower production costs and shorter development cycles. Second, mRNA technology can efficiently regulate immune responses and can induce antibodies with higher affinity. Compared with traditional immunization methods, mRNA immunization can avoid the complexity of protein expression and can directly synthesize antigens in host cells, reducing possible errors in the production process. Finally, the mRNA immunization method is highly customizable and can adjust the immunization strategy according to different needs (such as antigen type, immune strength, etc.), and has strong scalability.
How to evaluate the affinity and specificity of antibodies produced by mRNA immunization?

The evaluation of the affinity and specificity of antibodies produced by mRNA immunization is mainly done through in vitro experiments. First, ELISA (enzyme-linked immunosorbent assay) can be used to detect whether the antibody can specifically bind to the target antigen, and the affinity can be evaluated by measuring the half-maximal inhibitory concentration (IC50). Second, the binding kinetics between the antibody and the target cell or target antigen can be analyzed by flow cytometry or surface plasmon resonance (SPR) to further confirm its affinity and binding constant. For specificity assessment, in addition to specific binding to the target, it is also necessary to ensure that the antibody does not produce cross-reactions by testing other non-target molecules. In addition, functional experiments, such as neutralization experiments, can be used to detect the biological activity of antibodies in actual applications.

What is the mRNA immune antibody preparation service? What are its main applications?

The mRNA immune antibody preparation service is an innovative method to achieve antibody immunity through mRNA technology. By introducing mRNA encoding the target antigen into the host cell, it is expressed and displayed in the body, thereby stimulating the immune system to produce antibodies. This service is often used in areas such as antibody development, vaccine research, and antibody drug discovery. Compared with traditional protein immunization methods, mRNA immunization has higher flexibility and scalability, and can quickly respond to the needs of new targets. In terms of application, mRNA immunization can be used to quickly generate antibodies against specific pathogens or tumors, and is widely used in antibody development in infectious diseases, cancer immunotherapy, and allergy immunity.
What is the basic principle of mRNA immune antibody preparation?

The basic principle of mRNA immune antibody preparation is to use mRNA as a gene expression vector to deliver mRNA encoding the target antigen to host cells (such as in vivo or in vitro cells of mice, rabbits, or other experimental animals). After receiving the mRNA, the host cell uses its inherent translation mechanism to synthesize the target antigen. After the immune system recognizes these synthesized antigen molecules, it will produce specific antibodies. By regularly sampling and analyzing the immune response in animals, antibodies with high affinity and specificity for the target antigen can be screened. mRNA immunization provides a more efficient and flexible way to produce antibodies by simulating the natural immune process of pathogen infection.
How does mRNA immunization antibody preparation service differ from traditional antibody preparation methods?

mRNA immunization antibody preparation service has multiple advantages over traditional antibody preparation methods (such as protein immunization and cell immunization). First, mRNA immunization does not require cloning and expression of antigen proteins, avoiding the common difficulties and costs in protein production. Second, mRNA immunization can directly guide the production of target antigens in animals, and the immune response is closer to the natural physiological state, so it may produce antibodies with higher affinity and specificity. In addition, mRNA immunization is more time-efficient, and preliminary immune response results can usually be obtained within a few weeks, while traditional methods may take months. In addition, mRNA immunization is more flexible and scalable, which is particularly important for rapid response to emerging diseases or antibody development for undiscovered targets.
What are the advantages of mRNA immunization antibody preparation?

The advantages of mRNA immunization antibody preparation are mainly reflected in its efficiency, flexibility and cost-effectiveness. First, mRNA immunization can quickly respond to different targets and is particularly suitable for antibody development for emerging diseases. Since there is no need to produce large amounts of protein or cell culture, this method has lower production costs and shorter development cycles. Second, mRNA technology can efficiently regulate immune responses and can induce antibodies with higher affinity. Compared with traditional immunization methods, mRNA immunization can avoid the complexity of protein expression and can directly synthesize antigens in host cells, reducing possible errors in the production process. Finally, the mRNA immunization method is highly customizable and can adjust the immunization strategy according to different needs (such as antigen type, immune strength, etc.), and has strong scalability.
How to evaluate the affinity and specificity of antibodies produced by mRNA immunization?

The evaluation of the affinity and specificity of antibodies produced by mRNA immunization is mainly done through in vitro experiments. First, ELISA (enzyme-linked immunosorbent assay) can be used to detect whether the antibody can specifically bind to the target antigen, and the affinity can be evaluated by measuring the half-maximal inhibitory concentration (IC50). Second, the binding kinetics between the antibody and the target cell or target antigen can be analyzed by flow cytometry or surface plasmon resonance (SPR) to further confirm its affinity and binding constant. For specificity assessment, in addition to specific binding to the target, it is also necessary to ensure that the antibody does not produce cross-reactions by testing other non-target molecules. In addition, functional experiments, such as neutralization experiments, can be used to detect the biological activity of antibodies in actual applications.

Consult Now mRNA Antibody Production Services

Animal Immunization Technology Services

█ mRNA Immunization Service Procedure

█ mRNA Immunology Services (Using Nanobodies as an Example)

█ Advantages of mRNA Immunological Services

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mRNA Antibody Production Services Frequently Asked Questions

What is the mRNA immune antibody preparation service? What are its main applications?

What is the basic principle of mRNA immune antibody preparation?

How does mRNA immunization antibody preparation service differ from traditional antibody preparation methods?