pH-Dependent Antibody Development Services-Tekbiotech-Yeast and Phage Display CRO, Expert in Nano-body and Antibody Drug Development

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pH-Dependent Antibody Development Services

pH-dependent antibodies, also known as pH-switchable (acid-switched) antibodies, are capable of modulating their binding affinity to target antigens in response to changes in the environmental pH and represent one of the key research focuses in the fields of cancer therapy and autoimmune/metabolic diseases. Designing and screening pH-dependent antibodies that bind with high affinity in the acidic tumor microenvironment (pH ~6.5) and with low affinity under normal physiological conditions (pH 7.4) enables selective binding at tumor sites, thereby reducing toxicity to normal tissues—a development of significant importance for cancer therapy. Furthermore, the design and screening of pH-dependent antibodies that bind their antigens tightly in the blood (pH 7.4) but release them within endosomes (acidic pH ~6.0)—utilizing the FcRn-mediated antibody recycling mechanism in vivo—can extend the half-life of pH-dependent antibody drugs and enhance antigen clearance, thereby reducing the frequency and dose of administration. This is also of great significance for long-term treatment with antibody drugs.

TekBiotech has been dedicated to antibody development and research for many years and is able to provide clients with high-quality pH-dependent antibody development services. TekBiotech has accumulated extensive experience in the development of pH-dependent antibodies. Based on proven yeast display technology and customized FACS screening strategies, the company has established a comprehensive pH-dependent antibody discovery platform, enabling it to provide clients with pH-dependent antibody development services for a wide range of species (including mice, rabbits, sheep, goats, cattle, and camelids) and types (including Fab, scFv, and VHH). In addition, TekBiotech can customize appropriate pH-dependent antibody development and detection protocols based on clients’ downstream detection requirements to address challenges encountered during project development.

█ pH-Dependent Antibody Development Services

pH-dependent antibodies are antibodies that bind with high affinity under specific pH conditions and with low affinity under other pH conditions. Screening and detection strategies vary depending on project requirements and experimental objectives. Using yeast display technology, TekBiotech is able to provide clients with comprehensive, integrated services for pH-dependent antibodies. We construct various types of yeast-display antibody libraries (e.g., yeast-display Fab libraries, yeast-display scFv libraries, yeast-display VHH libraries) from target antibody gene libraries (e.g., antibody gene libraries derived from animal immunization, or oligo pools with engineered mutations at specific antibody sites) according to client requirements (effective library size: 10?–10?, library diversity >85%, library insertion rate/positive rate > 85%). We also design optimized FACS screening protocols based on the characteristics of the target antibody (customized screening strategies under different pH conditions) to obtain high-quality pH-dependent antibodies suitable for downstream experimental applications. Additionally, leveraging TekBiotech’s recombinant protein fermentation platform, we can scale up antibody production to meet clients’ needs for large-scale antibody production. TekBiotech’s pH-dependent antibody development process is as follows:

Figure 1: The procedure for developing pH-dependent antibodies using yeast display technology.

█ Types of pH-Dependent Antibody Development Services

TekBiotech provides high-quality, one-stop pH-dependent antibody development services, covering every stage from early-stage antigen design and synthesis, animal immunization, antibody library construction using yeast display technology, customized antibody library flow cytometry (FACS) screening, and in vitro detection, all the way through to antibody process scale-up. In addition, leveraging Trimer Codon technology, TekBiotech provides clients with high-quality synthesis services for oligonucleotide pools containing site-specific mutations in antibodies, meeting their application needs for downstream library construction and screening.

TekBiotech’s pH-dependent antibody development services fall into the following two categories:

Antibody Type	pH-Dependent Antibodies Against Tumors	pH-Dependent Antibodies for Autoimmune/Metabolic Diseases
Key Advantages	Tumor-selective binding reduces toxicity to normal tissues	Enhanced antigen clearance, extended antibody half-life, and reduced dosing frequency and dose

█ Advantages of pH-Dependent Antibody Development Services


One-Stop Solution	Customized Screening Solutions	Development of Antibodies Across Multiple Species	Mature Downstream Detection Platforms
Comprehensive services ranging from early-stage antigen design and synthesis, antibody screening, and detection to process scale-up	Diverse screening protocols for different pH-dependent antibodies	A wide variety of species are available, including mice, rabbits, sheep, goats, cattle, and camelids (alpaca, llama, and camel).	In vitro binding assays, cellular-level assays, etc.

Various pH-Dependent Antibodies	High-Quality Yeast Library	A Variety of Antibody Libraries are Available	High-Quality Oligo Pool Synthesis Services
Development of pH-dependent antibodies targeting tumors and pH-dependent antibodies associated with autoimmune and metabolic diseases, among others	Effective library size: 10?–10?; library diversity > 85%; library insertion rate/positive rate > 85%	Fab antibody libraries, scFv antibody libraries, VHH antibody libraries, etc.	Proven Trimer Codon technology for the synthesis of high-quality oligo pools